Methioninase (rMETase) Publications

1.       Hoffman, R.M. and Erbe, R.W.  High in vivo rates of methionine biosynthesis in transformed human and malignant rat cells auxotrophic for methionine.  Proc. Natl. Acad. Sci. USA 73, 1523-1527, 1976.[PDF]

2.       Hoffman, R.M., Jacobsen, S.J., and Erbe, R.W.  Reversion to methionine independence by malignant rat and SV40-transformed human fibroblasts.  Biochem. Biophys. Res. Commun. 82, 228-234, 1978.[PDF]

3.       Hoffman, R.M., Jacobsen, S.J., and Erbe, R.W. Reversion to methionine independence in simian virus 40-transformed human and malignant rat fibroblasts is associated with altered ploidy and altered properties of transformation. Proc. Natl. Acad. Sci. USA 76, 1313-1317, 1979.[PDF]

4.        Jacobsen, S.J., Hoffman, R.M., and Erbe, R.W.  Regulation of methionine adenosyltransferase in normal diploid and SV40-transformed human fibroblasts.  J. Natl. Cancer Inst. 65, 1237-1244, 1980.[PDF]

5.        Hoffman, R.M., and Jacobsen, S.J.  Reversible growth arrest in simian virus 40-transformed human fibroblasts. Proc. Natl. Acad. Sci. USA 77, 7306-7310, 1980.[PDF]  

6.        Diala, E.S., Plent, M.M., Coalson, D.W. and Hoffman, R.M.  DNA methylation in normal and SV40-transformed human fibroblasts.  Biochem. Biophys. Res. Commun. 102, 1379-1384, 1981.[PDF]  

7.        Coalson, D.W., Mecham, J.O., Stern, P.H. and Hoffman, R.M.  Reduced availability of endogenously synthesized methionine for S-adenosylmethionine formation in methionine dependent cancer cells.  Proc. Natl. Acad. Sci. USA 79, 4248-4251, 1982.[PDF]

8.        Diala, E.S. and Hoffman, R.M.  Hypomethylation of HeLa cell DNA and the absence of 5-methylcytosine in SV40 and adenovirus (type 2) DNA: analysis by HPLC.  Biochem. Biophys. Res. Commun. 107, 19-26, 1982.[PDF]

9.        Hoffman, R.M.  Methionine dependence in cancer cells – A review.  In Vitro 18, 421-428, 1982.[PDF]

10.      Diala, E.S. and Hoffman, R.M.  DNA methylation levels in normal and chemically transformed mouse 3T3 cells. Biochem. Biophys. Res. Commun. 104, 1489-1494, 1982.[PDF]  

11.      Diala, E.S., Cheah, M.S.C., Rowitch, D., and Hoffman, R.M.  Extent of DNA methylation in human tumor cells. J. Natl. Cancer Inst. 71, 755-764, 1983.[PDF]  

12.     Mecham, J.O., Rowitch, D., Wallace, C.D., Stern, P.H. and Hoffman, R.M.  The metabolic defect of methionine dependence occurs frequently in human tumor cell lines.  Biochem. Biophys. Res. Commun. 117, 429-434, 1983.[PDF]  

13.     Oden, K.L., Carson, K., Mecham, J.O., Hoffman, R.M. and Clarke, S.  S-adenosylmethionine synthetase in cultured normal and oncogenically-transformed human and rat cells.  Biochem. Biophys. Acta  870, 270-277, 1983.[PDF]

14.      Stern, P.H., Mecham, J.O., Wallace, C.D. and Hoffman, R.M.  Reduced free-methionine in methionine-dependent SV40-transformed human fibroblasts synthesizing apparently normal amounts of methionine.  J. Cell Physiol. 117, 9-14, 1983.[PDF]

15.       Cheah, M.S.C., Wallace, C.D., and Hoffman, R.M.  Hypomethylation of DNA in human cancer cells: a site-specific change in the c-myc oncogene.  J. Natl. Cancer Inst. 73, 1057-1065, 1984.[PDF]

16.       Hoffman, R.M.  Altered methionine metabolism, DNA methylation and oncogene expression in carcinogenesis: a review and synthesis.  Biochem. Biophys. Acta, Reviews on Cancer 738, 49-87, 1984.[PDF]

17.       Stern, P.H. and Hoffman, R.M.  Elevated overall rates of transmethylation in cell lines from diverse human tumors. In Vitro – Rapid Commun. in Cell Biology 20, 663-670, 1984.[PDF]

18.       Stern, P.H., Wallace, C.D., and Hoffman, R.M.  Altered methionine metabolism occurs in all members of a set of diverse human tumor cell lines.  J. Cell Physiol. 119, 29-34, 1984.

19.       Hoffman, R.M.  Altered methionine metabolism and transmethylation in cancer.  Anticancer Research 5, 1-30, 1985.[PDF]

20.      Hoffman, R.M. and Stern, P.H.  Cancer, methionine and transmethylation.  In Biological Methylation and Drug Design, Borchardt, R.T., Creveling, C.R., and Ueland, P.M., eds., Clifton, New Jersey, The Human Press Inc., 215-225, 1986.  

21.       Stern, P.H. and Hoffman, R.M.  Enhanced in vitro selective toxicity of chemotherapeutic agents for human cancer cells based on a metabolic defect.  J. Natl. Cancer Inst. 76, 629-639, 1986.[PDF]  

22.      Hoffman, R.M.  Altered methionine metabolism and unbalanced methylation: a possible basis for the dynamic phenotype of cancer.  Absorption and Utilization of Amino Acids, M. Friedman, ed., CRC Press, Boca Raton, Florida, 1-7, 1989.

23.       Hoffman, R.M.  Unbalanced transmethylation and the perturbation of the differentiated state leading to cancer. BioEssays 12, 163-166, 1990.

24.       Guo, H.Y., Herrera, H., Groce, A., and Hoffman, R.M.  Expression of the biochemical defect of methionine dependence in fresh patient tumors in primary histoculture.  Cancer Res. 53, 2479-2483, 1993.[PDF]

25.       Guo, H.Y., Herrera, H., and Hoffman, R.M.  Unchecked DNA synthesis and blocked cell division induced by methionine deprivation in a human prostate cancer cell line.  In Vitro Cell. Dev. Biol. 29A, 359-361, 1993.[PDF] 

26.      Lishko, V.K., Lishko, O.V., and Hoffman, R.M.  The preparation of endotoxin-free l-methionine-a-deamino-g-mercaptomethane-lyase (l-methioninase) from Pseudomonas putida.  Protein Expression and Purification 4, 529-533, 1993.[PDF] 

27.       Lishko, V.K., Lishko, O.V., and Hoffman, R.M.  Depletion of serum methionine by methioninase in mice. Anticancer Research 13, 1465-1468, 1993.[PDF]   

28.       Guo, H., Lishko, V., Herrera, H., Groce, A., Kubota, T., and Hoffman, R.M.  Therapeutic tumor-specific cell-cycle block induced by methionine starvation in vivo.  Cancer Res. 53, 5676-5679, 1993.[PDF]   

29.       Hoshiya, Y., Guo, H., Kubota, T., Inada, T., Asenuma, F., Yamada, Y., Koh, J., Kitajima, M., and Hoffman, R.M. Human tumors are methionine dependent in vivo.  Anticancer Research 15, 717-718, 1995.[PDF] 

30.      Goseki, N., Yamazaki, S., Shimojyu, K., Kando, F., Maruyama, M., Endo, M., Koike, M., and Takahashi, H. Synergistic effect of methionine-depleting total parenteral nutrition with 5-fluorouracil on human gastric cancer: A randomized, prospective clinical trial.  Jpn. J. Cancer Res. 86, 484-489, 1995.[PDF]   

31.       Tan, Y., Xu, M., Guo, H., Sun, X., Kubota, T., and Hoffman, R.M.  Anticancer efficacy of methioninase in vivo. Anticancer Research 16, 3931-3936, 1996.[PDF]   

32.       Tan, Y., Zavala Sr., J., Xu, M., Zavala Jr., J., and Hoffman, R.M.  Serum methionine depletion without side effects by methioninase in metastatic breast cancer patients.  Anticancer Research 16, 3937-3942, 1996.[PDF]  

33.       Guo, H., Tan, Y., Kubota, T., Moossa, A.R., and Hoffman, R.M.  Methionine depletion modulates the antitumor and antimetastatic efficacy of ethionine.  Anticancer Research 16, 2719-2724, 1996.[PDF]   

34.       Hoshiya, Y., Kubota, T., Matsuzaki, S., Kitajima, M., and Hoffman, R.M.  Methionine starvation modulates the efficacy of cisplatin on human breast cancer in nude mice.  Anticancer Research 16, 3515-3518, 1996.[PDF]

35.       Goseki, N., et al.  Evaluation of the survival time of A0-90 clinical trial for progressive or recurrent gastric cancer.  J. Jpn. Soc. for Cancer Ther., Vol. XXXI, No. 8, p. 610 (172), 20 September 1996.[PDF]

36.       Tan, Y., Xu, M., Tan, X-Z., Tan, X-Y., Wang., X., Saikawa, S., Nagahama, T., Sun, X., Lenz, M., and Hoffman, R.M.  Overexpression and large-scale production of recombinant l-methionine-a-deamino-g- mercaptomethane-lyase for novel anticancer therapy.  Protein Expression and Purification 9, 233-245, 1997. [PDF] 

37.       Hoffman, R.M.  Recombinant Methioninase.  Drugs of the Future 22, 130-134, 1997.[PDF]

38.       Hoffman, R.M.  Methioninase: A therapeutic for diseases related to altered methionine metabolism and transmethylation: cancer, heart disease, obesity, aging, and Parkinson’s disease.  Human Cell 10, 69-80, 1997.[PDF]

39.      Tan, Y., Zavala, J. Sr., Han, Q., Xu, M., Sun, X., Tan, X-H., Tan, X., Magana, R., Geller, J., and Hoffman, R.M. Recombinant methioninase infusion reduces the biochemical endpoint of serum methionine with minimal toxicity in high-stage cancer patients.  Anticancer Research 17, 3857-3860, 1997.[PDF]

40.      Hoffman, R.M. Is methioninase useful for the prevention of hyperhomocysteinemia-associated cardiovascular disease? Homocysteine Metabolism: From Basic Science to Clinical Medicine. Ed: I. Graham, H. Refsum, I.H. Rosenberg, P.M. Ueland, Kluwer Academic Publishers, Massachusetts, pp. 155-156, 1997.[PDF]  

41.      Kokkinakis, D.M., Schold, S.C. Jr., Hori, H., and Nobori, T.  Effect of long-term depletion of plasma methionine on the growth and survival of human brain tumor xenografts in athymic mice.  Nutrition and Cancer 29, 195-204, 1997.[PDF]

42.      Kokkinakis, D.M., von Wronski, M.A., Vuong, T.H., Brent, T.P., and Schold, S.C. Jr.  Regulation of O6-methylguanine-DNA methyltransferase by methionine in human tumour cells.  British Journal of Cancer 75, 779-788, 1997.[PDF]

43.      Hoshiya, T., Kubota, T., Inada, T., Kitajima, M., and Hoffman, R.M.  Methionine-depletion modulates the efficacy of 5-fluorouracil on human gastic cancer in nude mice.  Anticancer Research 17, 4371-4376, 1997.[PDF]  

44.     Tan, Y., Sun, X., Xu, M., An, Z., Tan, X-Z., Tan, X-Y., Han, Q., Miljkovic, D.A., Yang, M., and Hoffman, R.M. Polyethylene glycol conjugation of recombinant methioninase for cancer therapy.  Protein Expression and Purification 12, 45-52, 1998. [PDF] 

45.      Nagahama, T., Goseki, N., and Endo, M.  Doxorubicin and vincristine with methionine depletion contributed to survival in the Yoshida sarcoma bearing rats.  Anticancer Research 18, 25-32, 1998.[PDF]

46.     Yoshioka, T., Wada, T., Uchida, N., Maki, H., Yoshida, H., Ide, N., Kasai, H., Hojo, K., Shono, K., Maekawa, R., Yagi, S., Hoffman, R.M., and Sugita, K.  Anticancer efficacy in vivo and in vitro, synergy with 5-fluorouracil, and safety of recombinant methioninase.  Cancer Research 58, 2583-2587, 1998.[PDF]

47.      Tan, Y., Sun, X., Xu, M., Tan, X-Z., Sasson, A., Rashidi, B., Han, Q., Tan, X-Y., Wang, X., An, Z., Sun,  F-X., and Hoffman, R.M.  Efficacy of recombinant methioninase in combination with cisplatin on human colon tumors in nude mice.  Clinical Cancer Research 5, 2157-2163, 1999. [PDF] 

48.      Miki, K., Xu, M., An, Z., Wang, X., Yang, M., Al-Refaie, W., Sun, X., Baranov, E., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  Survival efficacy of the combination of the methioninase gene and methioninase in a lung cancer orthotopic model.  Cancer Gene Therapy 7, 332-338, 2000.[PDF]

49.      Miki, K., Al-Refaie, W., Xu, M., Jiang, P., Tan, Y., Bouvet, M., Zhao, M., Gupta, A., Chishima, T., Shimada, H., Makuuchi, M., Moossa, A.R., and Hoffman, R.M.  Methioninase gene therapy of human cancer cells is synergistic with recombinant methioninase treatment. Cancer Research 60, 2696-2702, 2000. [PDF]

50.     Sridhar, V., Xu, M., Han, Q., Sun, X., Tan, Y., Hoffman, R.M., and Prasad, G.S.  Crystallization and preliminary crystallographic characterization of recombinant L-methionine-a-deamino-g-mrcaptomethane lyase (Methioninase). Acta Cryst., D56, 1665-1667, 2000.[PDF] 

51.     Machover, D., Zittoun, J., Broet, P., Metzger, G., Orrico, M., Goldschmidt, E., Schilf, A., Tonetti, C., Tan, Y., Delmas-Marsalet, B., Luccioni, C., Falissard, B., and Hoffman, R.M. Cytotoxic synergism of methioninase in combination with 5-fluorouracil and folinic acid. Biochem. Pharmacology 61, 867-876, 2001.[PDF]

52.      Kokkinakis, D.M., Hoffman, R.M., Frenkel, E.P., Wick, J.B., Han, Q., Xu, M., Tan, Y., Schold, S.C.  Synergy between methionine stress and chemotherapy in the treatment of brain tumor xenografts in athymic mice.  Cancer Research 61, 4017-4023, 2001. [PDF] 

53.     Miki, K, Xu, M., Gupta, A., Ba, Y., Tan, Y., Al-Refaie, W., Bouvet, M., Makuuchi, M., Moossa, A.R., and Hoffman, R.M.  Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate.  Cancer Research 61, 6805-6810, 2001. [PDF]

54.     Machover D., Zittoun J., Saffroy R., Broet P., Giraudier S., Magnaldo T., Goldschmidt E., Debuire B., Orrico M., Tan Y., Mishal Z., Chevallier O., Tonetti C., Jouault H., Ulusakaraya A., Tanguy M.-L., Metzger G., Hoffman R.M.  Treatment of Cancer Cells with Methioninase Produces DNA Hypomethylation and Increases DNA Synthesis. Cancer Research, 62, 4685-4689, 2002. [PDF]

55.      Li, S.,  Yang, Z., Sun, X., Tan, Y., Yagi, S., and Hoffman, R.M. A simultaneous colorimetric assay of free and protein-coupled polyethylene glycol. Anal. Biochem. 313, 335-337, 2003.[PDF]  

56.      Yamamoto, N., Gupta, A., Xu, M., Miki, K. Tsujimoto, Y., Tsuchiya, H., Tomita, K., Moossa, A.R., Hoffman, R.M. Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells. Cancer Gene Therapy 10, 445-450. 2003 [PDF] 

57.     Gupta, A., Miki, K., Xu, M., Yamamoto, N., Moosa, A.R., and Hoffman, R.M. Combination efficacy of doxorubicin and adenoviral methioninase gene therapy with prodrug selenomethionine. Anticancer Research 23, 1181-1188, 2003.[PDF]

58.     Hoffman, R.M. PEG-methioninase. In: Advances in Experimental Medicine and Biology: Polymer Drugs in the Clinical Stage, vol. 519, 69-79. Maeda, H., Kabanov, A., Kataoka, K., Okano, T., eds. Dordecht, The Netherlands: Kluwer Academic/Plenum Publishers, 2003. 

59.     Sun, X., Yang, Z., Li,S., Tan, Y., Zhang, N., Wang, X., Yagi, S., Yoshioka, T., Takimoto, A., Mitsushima, K., Suginaka, A., Frenkel, E.P., and Hoffman, R.M. In vivo efficacy of recombinant methioninase is enchanced by the combination of polyethylene glycol conjugation and pyridoxal 5' phosphate supplementation. Cancer Research, 63, 8377-8383, 2003. [PDF]

60.      Takakura, T., Mitsushima, K., Yagi, S., Inagaki, K., Tanaka, H., Esaki, N., Soda, K., Takimoto, A. Assay method for antitumor L-methionine g-lyase: comprehensive kinetic analysis of the complex reaction with L-methionine. Analytical Biochemistry 327, 233-240.2004. [PDF]

61.     Yang, Z., Wang, J., Yoshioka, T., Li, B., Lu, Q., Li, S., Sun, X., Tan, Y., Yagi, S., Frenkel, E.P., Hoffman, R.M. A. Pharmacokinetics, Methionine Depletion, and Antigenecity of Recombinant Methioninase in Primates. Clinical Cancer Research 10, 2131-2138, 2004. [PDF]

62.      Li, S-K., Yang, Z., Sun, X., Tan, Y., Yagi, S., and Hoffman, R.M. A. Protein carboxyl amidation increases the potential extent of protein polyethylene glycol conjugation. Analytical Biochemistry, 330, 264-271, 2004.[PDF]

63.     Yang, Z., Sun, X., Li, S., Tan, Y., Wang, X., Zhang, N., Yagi, S., Takakaura, T., Kobayashi, Y., Takimoto, A., Yoshioka, T., Suginaka, A., Frenkel, E.P., and Hoffman, R.M. Circulating half-life of PEGylated recombinant methioninase holoenzyme is highly dose dependent on cofactor pyridoxal-5'-phosphate. Cancer Research 64, 5775-5778, 2004. [PDF]

64.     Yang, Z., Wang, J., Lu, Q., Xu, j., Kobayashi, Y., Takakura, T., Takimoto, A., Yoshioka, T., Lian, C., Chen, C., Zhang, D., Zhang, Y., Li, S., Sun, X., Tan, Y., Yagi, S., Frenkel, E.P., and Hoffman, R.M. PEGylation confers greatly extended half-life and attenuated immunogenecity to recombinant methioninase in primates. Cancer Research 64, 6673-6678, 2004.[PDF]

65.     Sun, X., Tan, Y., Yang, Z., Li, S., and Hoffman, R.M. A rapid HPLC method for the measurement of ultra-low plasma methionine concentrations applicable to methionine depletion therapy. AntCancer Research 24, 59-62, 2005.[PDF]

66.     Takakura, T., Ito, T., Yagi, S., Notsu, Y., Itakura, T., Nakamura, T., Inagaki, K., Esaki, N., Hoffman, R.M., Takimoto, A. High-level expression and bulk crystallisation of recombinant L-methionine-g-lyase, an anticancer agent. Appl. Microbiol. Biotechnol. 70, 183-192, 2006. DOI: 10.1007/s00253-005-0038-2.[PDF]

67.     Takakura, T., Takimoto, A., Notsu, Yoshida, H., Ito, T., Nagatome, H., Ohno, M., Kobayashi, Y., Yoshika, T., Inagaki, K., Yagi, S., Hoffman, R.M., and Esaki, N. Physicochemical and pharmacocinetic characterization og highly potent recombinant L-methionine-g-lyase conjugated with polyethylene glycol as an antitumor agent. Cancer Res. 66, 2807-2814, 2006. [PDF]  

68.     Kudou, D., Misaki, S., Yamashita, M., Tamura, T., Takakura, T., Yoshioka, T., Yagi, S., Hoffman, R.M., Takimoto, A., Esaki, N., and Inagaki, K. Structure of the antitumour enzyme l-methionine g-lyase from Pseudomonas putida at 1.8Å resolution. J. Biochem. 141, 535-544, 2007.

69.     Al-Awadi, F., Yang, M., Tan, Y., Han, Q., Li, S., Hoffman, R.M. Human tumor growth in nude mice is associated with decreased plasma cysteine and homocysteine. Anticancer Research 28, 2541-2544, 2008.

70.     Tan, Y., Xu, M., and Hoffman, R.M. Broad selective efficacy of recombinant methioninase and polyethylene glycol-modified recombinant methioninase on cancer cells in vitro. Anticancer Res. 30, 1041-1046, 2010.

1.      Chishima, T., Miyagi, Y., Wang, X., Yamaoka, H., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression.  Cancer Res. 57, 2042-2047, 1997.

2.      Chishima, T., Miyagi, Y., Wang, X., Baranov, E., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Metastatic patterns of lung cancer visualized live and in process by green fluorescent protein expression.  Clinical & Experimental Metastasis 15, 547-552, 1997.

3.      Chishima, T., Miyagi, Y., Wang, X., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Visualization of the metastatic process by green fluorescent protein expression.  Anticancer Res. 17, 2377-2384, 1997.

4.      Chishima, T., Yang, M., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Shimada, H., Moossa, A.R., Penman, S., and Hoffman, R.M.  Governing step of metastasis visualized in vitro.  Proc. Natl. Acad. Sci. USA 94, 11573-11576, 1997.

5.      Chishima, T., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Yang, M., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Use of histoculture and green fluorescent protein to visualize tumor cell host interaction.  In Vitro Cell. Dev. Biol. 33, 745-747, 1997.

6.      Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression.  Cancer Res. 58, 4217-4221, 1998.

7.      Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M.  A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Res. 59, 781-786, 1999.

8.       Hoffman, R.M.  Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis.  Cancer and Metastasis Reviews 17, 271-277, 1999.

9.      Hoffman, R.M.  Green fluorescent protein to visualize cancer progression and metastasis.  Methods in Enzymology, Green Fluorescent Protein, Vol. 302. Ed: P. Michael Conn, Academic Press, San Diego, pp. 20-31, 1999.

10.  Naumov, G.N., Wilson, S.M., MacDonald, I.C., Schmidt, E.E., Morris, V.L., Groom, A.C., Hoffman, R.M., and Chambers, A.F.  Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis.  Journal of Cell Science 112, 1835-1842, 1999.

11.    Yang, M., Chishima, T., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Green fluorescent protein: A new light to visualize metastasis and angiogenesis in cancer.  Proc. of SPIE Conference on Molecular Imaging: Reporters, Dyes, Markers and Instrumentation 3500, 117-124, 1999.

12.    Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  Genetically fluorescent melanoma bone and organ metastasis models.  Clinical Cancer Res. 5, 3549-3559, 1999.

13.    Yang, M., Chishima, T., Wang, X., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. Multi-organ metastatic capability of Chinese hamster ovary cells revealed by green fluorescent protein (GFP) expression.  Clinical & Experimental Metastasis, 17, 417-422, 1999.

14.    Hoffman, R.M.  Visualization of metastasis in orthotopic mouse models with green fluorescent protein. In: Relevance of Tumor Models in Anticancer Drugs Development.  Eds.: Fiebig, H.H., Burger, A.M., Kluwver Academic Publishers, Dordrecht, The Netherlands, Vol. 54, 81-87, 1999.

15.    Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases.  Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000.

16.    Dove, A. Illuminating cancer models.  Nature Biotechnology 18, 261, 2000.

17.    Hoffman, R.M.  Correspondence re: Initial stages of tumor cell-induced angiogenesis: Evaluation via skin window chambers in rodent models. Journal of the National Cancer Institute 92, 1445, 2000.

18.    Yang, M., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M.  External optical imaging freely-moving mice with green fluorescent protein-expressing metastatic tumors.  Proc. of SPIE Conference, 3921, 256-259, 2000.

19.    Corrigendum, BioFeedback.  Biotechniques 29, 544, September 2000.

20.    Yang, M., Baranov, E., Moossa, A.R., Penman, S., Hoffman, R.M.  Visualizing gene expression by whole-body fluorescence imaging.  Proc. Natl. Acad. Sci. USA 97, 12278-12282, 2000.                          

21.    Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.

22.    Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M.  A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model.  Clinical & Experimental Metastasis 18, 57-60, 2000.

23.    Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E., Moossa, A.R., Hoffman, R.M.  Chronologically-specific metastatic targeting of human pancreatic tumors in orthotopic models.  Clinical & Experimental Metastasis 18, 213-218, 2000.

24.    Yang, M., Baranov, E., Li, X-M., Wang, J-W., Jiang, P., Li, L., Moossa, A.R., Penman, S., Hoffman, R.M.  Whole‑body and intravital optical imaging of angiogenesis in orthotopically implanted tumors.  Proc. Natl. Acad. Sci. USA 98, 2616-2621, 2001.

25.    Robinson, K.  Imaging system captures whole-body GFP images.  Biophotonics International, April, 54-55, 2001.

26.    Pfeifer, A., Kessler, T., Yang, M., Baranov, E., Kootstra, N., Cheresh, D.A., Hoffman, R.M., and Verma, I.M.  Transduction of liver cells by lentiviral vectors: Analysis in living animals.  Molecular Therapy 3, 319-322, 2001.

27.    Hoffman, Robert M.  Visualization of GFP-expressing tumors and metastasis in vivo.  BioTechniques 30, 1016-1026, 2001.

28.    Hutchinson, E.  Fluorescence imaging of tumour metastasis and angiogenesis.  The Lancet Oncology, 2, 254, 2001.

29.    McCann, J.  Jellyfish protein gives new glow to tumor imaging.  Journal of the National Cancer Institute 93, 976-977, 2001.  [HTML]

30.    Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M.  Antimetastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model.  Clinical & Experimental Metastasis 18, 379-384, 2001.

31.    Zhao, M., Yang.M., Baranov, E., Wang, X., Penman, S., Moossa, A.R., Hoffman, R.M. Spatial-temporal imaging of bacterial infection and antibiotic response in intact animals. Proc. Natl. Acad. Sci. USA 98, 9814-9818, 2001.

32.    Hoffman, R.M.   Green Fluorescent Protein for Metastasis Research.  In: Brooks, S., and Schumacher, U., eds. Metastasis Research Protocols. Vol. II. Analysis of Cell Behavior In Vitro and In Vivo.  Totowa, NJ: Humana Press Inc., 285-298, 2001.

33.    Hoffman, R.M.  GFP-Expressing Metastatic-Cancer Mouse Models.  In:  Teicher, B., ed.  Tumor Models in Cancer Research.  Totowa, NJ: Humana Press Inc., 99-112, 2002.

34.    Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H.  VEGF receptor antisense therapy inhibits angiogenesis and peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy 9, 197-201, 2002.

35.    Yang, M., Baranov, E., Wang, J-W., Jiang, P., Wang, X., Sun, F-X., Bouvet, M., Moossa, A.R., Penman, S., and Hoffman, R.M.  Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model.  Proc. Natl. Acad. Sci. USA 99, 3824-3829, 2002. 

36.    Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M.  Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model.  Cancer Research 62, 1534-1540, 2002.

37.    Hoffman, R.M.  Green fluorescent protein imaging of tumor cells in mice.  Lab Animal 31, 34-41, 2002.

38.    Bouvet, M., Nardin, S.R., Burton, D.W., Lee, N.C., Yang, M., Wang, X., Baranov, E., Behling, C., Moossa, A.R., Hoffman, R.M., and Deftos L.J.  Parathyroid hormone-related protein as a novel tumor marker in pancreatic adenocarcinoma.  Pancreas 24, 284-290, 2002.

39.    Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M. and Lowe, S.W.  Dissecting p53 tumor suppressor functions in vivo.  Cancer Cell 1, 289-298, 2002.

40.    Schmitt, C.A., Yang, M., Fridman, J.S., Baranov, E., Hoffman, R.M., and Lowe, S.W.  Senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy.  Cell 109, 335–346, 2002.

41.    Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Optically imageable metastatic model of human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002. 

42.    Hoffman, R.M.  Watching real-time metastasis in vivo.  Trends in Molecular Medicine, 8(7), 354-355, 2002.

43.    Hoffman, R.M.  Whole-body fluorescence imaging with Green Fluorescence Protein.  In: Hicks, B.W., ed.  Methods in Molecular Biology, Vol. 183: Green Fluorescent Protein: Applications and Protocols.  Totowa, NJ: Humana Press, 135-148, 2002.

44.    Hoffman, R.M.  In vivo imaging of metastatic cancer with fluorescent proteins.  Journal of Cell Death and Differentiation 9, 786-789, 2002. 

45.    Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman, R.M., Benedict, W.F. Visualizing superficial human bladder cancer cell growth in vivo by green fluorescent protein expression.  Cancer Gene Therapy 9, 681-686, 2002.

46.    Hoffman, R.M.  Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models.  Lancet Oncology 3, 546-556, 2002.

47.    Saito, N., Zhao, M., Li, L., Baranov, E., Yang, M., Ohta, Y., Katsuoka, K., Penman, S. and Hoffman, R. High efficiency genetic modification of hair follicles and growing hair shafts. Proc. Natl. Acad. Sci. 99, 13120-13124, 2002

48.    Sun, F-X., Tohgo, A., Bouvet, M., Yagi, S., Nassirpour, R., Moossa, A.R., and Hoffman, R.M.  Efficacy of camptothecin analog DX-8951f (Exatecan Mesylate) on human pancreatic cancer in an orthotopic metastatic model.  Cancer Research 63, 80-85, 2003.

49.    Wang, J-W., Yang, M., Wang, X., Sun, F-X., Li, X-M., Yagi, S., and Hoffman, R.M. Antimetastatic efficacy of oral 5-FU imaged by green fluorescent protein in real time.  Anticancer Research 23, 1-6, 2003.

50.    Yamamoto, N., Yang, M., Jiang, P., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases.  Clin. Exp. Metastasis 20, 181-185, 2003.

51.    John, C.M., Leffler, H., Kahl-Knutsson, B., Svensson, I., Jarvis, G.A. Truncated Galectin-3 Inhibits Tumor Growth and Metastasis in Orthotopic Nude Mouse Model of Human Breast Cancer. Clinical Cancer Res. 9, 3808-3814, 2003.

52.    Glinskii, A.B., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M., Glinsky, G.V. Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models. Cancer Res. 63, 4239-4243, 2003.

53.    Katz, M.H., Spivack, D.E., Takimoto, S., Fang, B., Burton, D.W., Moosa, A.R., Hoffman, R.M., and Bouvet, M. Gene therapy of pancreatic cancer with green fluorescent protein and tumor necrosis factor-related apoptosis-inducing ligand fusion gene expression driven by a human telomerase reverse transcriptase promoter. Annals of Surgical Oncology 10, 762-772, 2003.

54.    Katz, M., Takimoto, S., Spivack, D., Moossa, A.R., Hoffman, R.M., Bouvet, M. A novel red fluorescent protein orthotopic pancreatic cancer model for the preclinical  evaluation of chemotherapeutics. J.Surg. Res. 113, 151-160, 2003. 

55.    Katz, M.H., Bouvet, M., Takimoto, S., Spivac, D., Moossa, A.R., Hoffman, R.M. Selective antimetastatic activity of cytosine analog CS-682 in a red fluorescent protein orthotopic model of pancreatic cancer. Cancer Res. 63, 5521-5525, 2003.

56.    Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R.M., and Tarin, D. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from non-metastatic breast tumors revealed by labeling with green fluorescent protein. Clinical Cancer Res. 9, 3808-3814, 2003.

57.    Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Real-time imaging of individual color-coded metastatic colonies in vivo.  Clin. Exp. Metastasis 20(7), 633-638, 2003.

58.    Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging.  Cancer Research 63, 7785-7790, 2003.

59.   Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M.  Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells.  Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.

60.    Hoffman, R.M., and Yagi, S. In vivo single-cell imaging. Bio-venture (Japan) 3, 77-78, 2003.

61.    Mitsiades, C.S., Mitsiades, N.S., Bronson, R.T., Chauhan, D., Munshi, N., Treon, S.P., Maxwell, C.A., Pilarski, L., Hideshima, T., Hoffman, R.M., and Anderson, K.C. Fluorescence imaging of multiple myeloma cells in a clinically relevant SCID/NOD in vivo model: biologic and clinical implications. Cancer Research 63, 6689-6696, 2003.

62.    Katz, M., Spivac, D., Takimoto, S., Bouvet, M., Moosa, A.R., R.M. Hoffman. An imageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer. Clin. Exp. Metastasis 21, 7-12, 2004. 

63.    Katz, M.H., Bouvet, M., Takimoto, S., Spivack, D., Moossa, A.R., and Hoffman, R.M. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer. Cancer Research 64, 1828-1833, 2004.

64.    Hoffman, R. Multicolor in vivo imaging in mouse models of cancer. Preclinica. 2, 109-113, 2004.

65.    Robinson, K. Dual-color fluorescence imaging distinguishes tumor from host. Biophotonics Intl., January, 56-57, 2004.

66.    Mihich, E. and Kaelin, W. Fifteenth Annual Pezcoller Symposium: Molecular in vivo visualization of cancer cells. Cancer research 64, 2929-2933, 2004.

67.    Hoffman, R.M. In vivo imaging with fluorescent proteins: THe new cell biology. Acta Histochemica 106, 77-87, 2004.

68.    Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G.M., Moossa, A.R., and Hoffman, R.M. Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Research 64, 4251-4256, 2004.

69.     Yang, M., Amoh, Y., Li, L., Baranov, E., Wang, J-W., Jiang, P., Moossa, A.R., and Hoffman, R.M. Dual-color fluorescence imaging of tumor-host interaction with green and fluorescent proteins. In: Genetically Engineered and Optical Probes for Biomedical Applications II (Savitsky, A.P., Brovko, L.Y., Bornhop, D.J., Raghavachari, R., Achilefu, S.I., eds). Proceedings of SPIE Conference, 5329, 54-60, 2004.

70.    Laakkonen, P., Akerman, M.E., Biliran, H., Yang, M., Ferrer, F., Karpanen, T., Hoffman, R.M., Ruoslani, E. Antitumor activity of a homing peptide that targets tumor lymphatics and tumor cells. Proc. Natl. Acad. Sci. USA 101, 9381-9386, 2004.

71.    Wang, J-W., Yang, M., Yagi, S., and Hoffman, R.M. Oral 5-FU is a more effective antimetastatic agent than UFT. Anticancer Research 24, 1353-1360, 2004. 

72.    Amoh, Y., Li, L., Yang, M., Moosa, A.R., Katsuoka, K., Penman, S., and Hoffman, R.M., Nascent blood vessels in the skin arise from nestin-expressing hair follicle cells. Proc. Natl. Acad. Sci. USA 101, 13291-13295, 2004.

73.   Bobek, V. Plachy, J., Pinterova, D., Kolotsova, K., Boubelik, M., Jiang, P. P., Yang, M., and Hoffman R. M. Development of a green fluorescent protein metastatic-cancer chick-embryo drug-screen model. Clinical & Experimental Metastasis 21, 347-352, 2004.

74.    Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A.R., and Hoffman, R.M.  Transgenic nude mouse with ubiquitous green fluorescent protein _expression as a host for human tumors.  Cancer Research 64, 8651-8656, 2004. 

75.    Hoffman, R.M. Imaging tumor angiogenesis with fluorescent proteins. Acta Pathologica, Microbiologica et Immunologica Scandinavica 112, 441-449, 2004.

76.    Wang, J., Yang, M., and Hoffman, R.M. Visualizing portal vein metastatic trafficking to the liver with green fluorescent protein-expressing tumor cells. Anticancer Research 24, 3699-3702,2004.

77.    Yamamoto, N., Yang, M., Jiang, P., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies. Anticancer Research 24, 4067-4072, 2004.

78.   Bobek, V., Kolostova, K., Pinterov, D., Boubelik, M., Jiang, P., Yang, M., and Hoffman, R.M. Syngeneic lymph-node-targeting model of green fluorescent protein-expressing Lewis lung carcinoma. Clinical & Experimental Metastasis 21, 705-708, 2004.

79.    Zhao, M., Yang, M., Li, X-M., Jiang, P., Li, S., Xu, M., and Hoffman, R.M.  Tumor-targeting bacterial therapy with amino acid auxotrophs of GFP-expressing Salmonella typhimurium.  Proc. of Natl. Acad. Sci USA 102, 755-760, 2005.

80.    Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005. DOI: 10.1002/pros.20146.

81.   Yang, M., Jiang, P., Yamamoto, N., Li, L., Geller, J., Moossa, A.R., and Hoffman, R.M. Real-time whole-body imaging of an orthotopic metastatic prostate cancer model expressing rad fluorescent protein. The Prostate 62, 374-379,2005. DOI #10.1002/pros.20125.

82.    Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005.

83.    Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., Hoffman, R.M. Hair-follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin.  Cancer Research 65, 2337-2343, 2005. 

84.    Berezovskaya, O., Schimmer, A.D., Glinskii, A.B., Pinilla, C., Hoffman, R.M., Reed, J.C., and Glinky, G.V.  Increased _expression of apoptosis inhibitor protein XIAP contributes to resistance to anoikis of human prostate cancer metastasis precursor cells.  Cancer Research 65, 2378-2386, 2005.

85.    Amoh, Y., Li, L., Katsuoka, K., Penman, S., and Hoffman, R.M.  Multipotent nestin-positive, keratin-negative hair-follicle-bulge stem cells can form neurons.  Proc. Natl. Acad. Sci. USA 102, 5530-5534, 2005. 

86.    Yamauchi, K., Yang, M., Jiang, P., Yamamoto, N., Xu, M., Amoh, Y., Tsuji, K., Bouvet, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Real-time in vivo dual-color imaging of intracapillary cancer cell and nucleus deformation and migration.  Cancer Res. 65, 4246-4252, 2005.

87.    Hoffman, R.M.  Imaging of angiogenesis in vivo with fluorescent proteins: fluorescence imaging of angiogenesis.  In: Meadows, Gary G., EdIntegration/Interaction of Oncologic Growth.  Book Series: Kaiser, H., Ed.  Cancer Growth and Progression, 2nd Ed., Vol. 15, pp. 37-45.  Dordrecht, The Netherlands: Springer, 2005.  ISBN #1-4020-3413-X.

88.    Amoh, Y., Yang, M., Li, L., Reynoso, J., Bouvet, M., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis.  Cancer Res. 65, 5352-5357, 2005. 

89.   Hoffman, R.M. and Yang, M. Dual-color whole-body imaging in mice. Nature Biotechnology 23, 790-791, 2005.

90.   Yang, M., Luiken, G., Baranov, E., and Hoffman, R.M. Facile whole-body imaging of internal fluorescent tumors in mice with an LED flashlight. Bio Techniques 39, 170-172, 2005.

91.   Hoffman, R.M. Advantages of multi-color fluorescent proteins for whole-body and in in vivo cellular imaging. Journal of Biomedical Optics 10(4), 41202, AN #041202, 2005.

92.    Hoffman, R.M.  The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5, 796-806, 2005. 

93.    Bouvet, M., Spernyak, J., Katz, M.H., Mazurchuk, R.V., Takimoto, S., Bernacki, R., Rustum, Y.M., Moossa, A.R., and Hoffman, R.M. High correlation of whole- body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer. Cancer Research 65, 9829-9833, 2005.

94.    Amoh, Y., Li, L., Campillo, R., Kawahara, K., Katsuoka, K., Penman, S., and Hoffman, R.M. Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves. Proc. Natl. Acad. Sci. USA 102, 17734-17738,2005.

95.    Hoffman, R.M.  In vivo cell biology of cancer cells visualized with fluorescent proteins.  In:  “In vivo cellular and molecular imaging” for Current Topics in Developmental Biology, Vol. 70, pp. 121-144. Ahrens, Eric T., ed.  London: Elsevier Life Sciences, 2005.  DOI: 10.1016/S0070-2153(05)70006-5.

96.    Hemman, M.T., Bric, A., Teruya-Feldstein, J., Herbst, A., Nilsson, J.A., Cordon-Cardo, C., Cleveland, J.L., Tansey, W.P., Lowe, S.W. Evasion of the p53 tumour surveillance network by tumour-derived MYC mutants. Nature 436, 807-811, 2005.

97.    Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moossa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area. Cancer Res. 66, 303-306, 2006.

98.  Pilch, J., Brown, D.M., Komatsu, M., Järvinen, T., Yang, M., Peters, D., Hoffman, R.M., and Ruoslahti, E.  Peptides selected for binding to clotted plasma accumulate in tumor stroma and wounds.  Proc. Natl. Acad. Sci. USA 103, 2800-2804, 2006.

99. Glinsky, G.V., Glinskii, A.B., Berezovskaya, O., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M.  Dual-color-coded imaging of viable circulating prostate carcinoma cells reveals genetic exchange between tumor cells in vivo, contributing to highly metastatic phenotypes.  Cell Cycle 5, 191-197, 2006.

100. Tsuji, K., Yang, M., Jiang, P., Maitra, A., Kaushal, S., Yamauchi, K., Katz, M.D., Moossa, A.R.,    Hoffman, R.M, Bouvet, M.  Common bile duct injection as a novel method for establishing RFP-expressing human pancreatic cancer in nude mice.  Journal of the Pancreas 7, 193-199, 2006.

101. Yamauchi, K., Yang, M., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moosa, A.R.,  Bouvet, M., and Hoffman, R.M. Development of real-time subcellular dynamic multicolor imaging of cancer cell trafficking in live mice with a variable-magnification whole-mouse imaging system. Cancer Res. 66, 4208-4214, 2006. Yamauchi et al_Cancer Res_Supplemental Movie 1; Yamauchi et al_Cancer Res_Supplemental Movie 2; Yamauchi et al_Cancer Res_Supplemental Movie 3

102. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model.  Clinical Cancer Research 12, 2602-2606, 2006.

103. Amoh, Y., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M.  Dual-color  imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine.  J. Surgical Research 132, 164-169, 2006.

104. Jiang, P., Yamauchi, K., Yang., Tsuji, K., Xu, M., Maitra, A., Bouvet, M., and Hoffman, R.M. Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell Cycle 5, 1198-1201, 2006.

105. Seitz, G., Warmann, S.W., Fuchs, J., Mau-Holzmann U.A., Ruck, P., Heitmann, H., Hoffman, R.M., Mahrt, J., Muller, G.A., and Wessels, J.T.  Visualization of xenotransplanted human rhabdomyosarcoma after transfection with red fluorescent protein.  Journal of Pediatric Surgery 41, 1369-1376, 2006.

106. Hoffman, R.M., and Yang, M.  Subcellular imaging in the live mouse.  Nature Protocols 1, 775-782, 2006.

107. Hoffman, R.M., and Yang, M.  Color-coded fluorescence imaging of tumor-host interactions.  Nature Protocols 1, 928-935, 2006.

108. Hoffman, R.M., and Yang, M.  Whole-body imaging with fluorescent proteins.  Nature Protocols 1, 1429-1438, 2006.

109.  Zhao, M., Yang, M., Ma, H., Li, X., Tan, X., Li, S., Yang, Z., and Hoffman, R.M.  Targeted therapy with a Salmonella typhimurium leucine-arginine auxotroph cures orthotopic human breast tumors in nude  mice.  Cancer Research 66, 7647-7652, 2006.

110.  Berezovska, O.P., Glinskii, A.B., Yang, Z., Li, X.M., Hoffman, R.M., and Glinsky, G.V.  Essential role for activation of the Polycomb Group (PcG) protein chromatin silencing pathway in metastatic prostate cancer.  Cell Cycle 5, 1886-1901, 2006.

111. Hoffman, R.M.  Real-time subcellular imaging in live animals:  New visible targets for cancer drug discovery.  IDrugs 9, 632-635, 2006.

112.  Amoh, Y., Nagakura, C., Maitra, A., Moosa, A.R., Katsuoka, K., Hoffman, R.M. and Bouvet, M. Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.

113. Bouvet, M., Tsuji, K., Yang, M., Jiang, P., Moossa, A.R., and Hoffman, R.M.  In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases.  Cancer Research 66, 11293-11297, 2006.

114. Hoffman, R.M.  Multi-color fluorescence imaging of cellular dynamics in vivo.  Proc. of SPIE 6098, 60980D1-60980D7, 2006.

115. Amoh, Y., Bouvet, M., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice.  Clin. Exp. Metastasis 23(7-8), 315-322, 2006.

116.  Hoffman, R.M., and Zhao, M. Whole-body imaging of bacterial infection and antibiotic response. Nature Protocols 1(6), 2988-2994, 2007. 

117. Hoffman, R.M.  AngioMouse: imageable models of angiogenesis.  In:  Angiogenesis Assays: A critical appraisal of current techniques, pp. 293-310.  Staton, C.A., Lewis, C., and Bicknell, R., eds.  Wiley and Sons, 2006.

118. Hoffman, R.M.  Subcellular imaging tracks cancer cells in mice.  SPIE Newsroom, 2006.  DOI: 10.117/2.1200608.0335.

119. Amoh, Y., Li, L., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Chemotherapy targets the hair-follicle vascular network but not the stem cells.  J. Invest. Dermatol. 127, 11-15, 2007.

120. Simberg, D., Duza, T., Park, J.H., Essler, M., Pilch, J., Zhang, L., Derfus, A.M., Yang, M., Hoffman,  R.M., Bhatia, S., Sailor, M.J., and Ruoslahti, E. Biomimetic amplification of nanoparticle homing to tumors. Proc. Natl. Acad. Sci. USA 104, 932-936, 2007. 

121. Seitz, G., Warmann, S.W., Heitmann, H., Ruck, P., Hoffman, R.M., Jörg Fuchs, Wessels, J.T.  Photodynamic therapy of rhabdomyosarcoma.  Int. J. Oncol. 30, 615-620, 2007

122. Yang, M., Jiang, P., Hoffman, R.M.  Whole-body subcellular multicolor imaging.  Proc. of SPIE 6449, 64490V1-64490V9, 2007.

123. Bouvet, M., Hoffman R.M.  Preclinical fluorescent mouse models of pancreatic cancer.  Proc. of SPIE 6449, 64490X1-64490X11, 2007.

124. Amoh, Y., Li, L., Katsuoka, K., Bouvet, M., and Hoffman, R.M.  GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors.  BioTechniques 42, 294-298, 2007.

125. Hoffman, R.M.  Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer.  Cancer 109, 1213-1214, 2007.

126.  Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M.  The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer.  Anticancer Res. 27, 713-718, 2007.

127. Yang, M., Jiang, P., and Hoffman, R.M.  Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time.  Cancer Res. 67 , 5195-5200, 2007.

128.  Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M.  Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma.  J. Surg. Res. 140, 165-170, 2007.

129. Zhao, M., Geller, J., Ma, H., Yang, M., Penman, S., Hoffman, R.M.  Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer.  Proc. Natl. Acad. Sci. USA 104, 10170-10174, 2007.

130. Guo, X-N., Rajput, A., Rose, R., Hauser, J., Beko, A., Kuropatwinski, K., LeVea, C., Hoffman, R.M., Brattain, M.G., and Wang, J.  Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model.  Cancer Res. 67, 5851-5858, 2007.

131. Liu, T., Ding, Y., Xie, W., Li, Z., Bai, X., Li, X., Fang, W., Ren, C., Wang, S., Hoffman, R.M., and Yao, K.  An imageable metastatic treatment model of nasopharyngeal carcinoma.  Clin. Cancer Res. 13, 3960-3967, 2007.

132. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M.  A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo.  Neoplasia 9, 625-633, 2007.

133. Hayashi, K., Jiang, P., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M.  Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels.  Cancer Res. 67, 8223-8228, 2007.

134. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Imaging of nucleolar dynamics during the cell cycle of cancer cells in live mice. Cell Cycle 6, 2706-2708, 2007. 

135. Hoffman, R. M. Subcellular imaging of cancer calls in live mice. In: Reporter Genes: A Practical Guide, Voll. 411, pp. 121-130, Methods in Molecular Biology series, Anson, D.S., editor. Humana Press, 2007. 

136. Hoffman, R.M. A better fluorescent protein for whole-body imaging. Trends in Biotechnology 26, 1-3, 2008.

137. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008.

138. Hoffman, R.M. Imaging in mice with fluorescent proteins: from macro to subcellular. Sensors 8, 1157-1173, 2008.

139. Seitz, G., Warmann, S.W., Fuchs, J., Heitmann, H., Mahrt, J., Busse, A-C., Ruck, P., Hoffman, R.M., and Wessels, J.T. Imaging of cell trafficking and metastasis of paediatric rhabdomyosarcoma. Cell Proliferation 41, 365-374, 2008.

140. Rajput, A., Dominguez San Martin, I., Rose, R., Beko, A., Levea, C., Sharratt, E., Mazurchuk, R., Hoffman, R.M., Brattain, M.G., and Wang, J. Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008.

141. Hoffman, R.M.  In vivo real-time imaging of nuclear-cytoplasmic dynamics of dormancy, proliferation and death of cancer cells.  Acta Pathologica, Microbiologica et Immunologica Scandinavica 116, 716-729, 2008.

142. Hoffman, R.M. Use of GFP for in vivo imaging: concepts and misconceptions. Proc. of SPIE 6868. 68680E1-68680E7, 2008.

143. Hoffman, R.M., Kishimoto, H., and Fujiwara, T. Specific in vivo labeling with GFP retroviruses, lentiviruses, and adenoviruses for imaging. Proc. of SPIE 6868. 68680F1-68680F6, 2008.

144. Hoffman, R.M., Hayashi, K., and Zhao, M. Therapeutic targeting of tumors with imageable GFP-expressing Salmonella typhimurium auxotrophic mutants. Proc. of SPIE 6868. 68680K1-68680K7, 2008.

145. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Imaging enhancement of malignancy by cyclophosphamide: surprising chemotherapy opposite effects. Proc. of SPIE 6868. 68680I1-68680I5, 2008.

146. Yang, M., Jiang, P., Al-Zaid, M., and Hoffman, R.M. Non-invasive imaging in vivo with fluorescent proteins from centimeters to micrometers. Proc. of SPIE 6868. 68680G1-68680G7, 2008.

145. McElroy, M., Hayashi, K., Kaushal, S., Bouvet, M., and Hoffman, R.M. Subcellular real-time in vivo imaging of intralymphatic and intravascular cancer-cell trafficking. Proc. of SPIE 6868. 68680J1-68680J6, 2008.

148. Arrach, N., Zhao, M., Porwollik, S., Hoffman, R.M., and McClelland, M. Salmonella promoters preferentially activated inside tumors. Cancer Res. 68, 4827-4832, 2008.

149. McElroy, M., Kaushal, S., Bouvet, M., Hoffman, R.M. Color-coded imaging of splenocyte-pancreatic cancer cell interactions in the tumor microenvironment. Cell Cycle 7, 2916-2921, 2008.

150. McElroy, M., Kaushal, S., Luiken, G., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Imaging of primary and metastatic pancreatic cancer using a fluorophore-conjugated anti-CA19-9 antibody for surgical navigation. World Journal of Surgery 32, 1057-1066, 2008.

151. Johnson, T.E., Luiken, G.A., Quigley, M.M., Xu, M., and Hoffman, R.M. Induced fluorescence of medullary carcinoma of the thyroid in vivo: A technology with potential to improve visualization of malignant tissue at the time of surgical resection. Ear Nose Throat Journal 87(8), E1, 2008.

152. Kaushal, S., McElroy, M.K., Luiken, G.A., Talamini, M.A., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Fluorophore-conjugated anti-CEA antibody for the intraoperative imaging of pancreatic and colorectal cancer. J. Gastrointest. Surg. 12, 1938-1950, 2008.

153. McElroy, M., Bouvet, M., and Hoffman, R.M. Color-coded fluorescent mouse models of cancer cell interactions with blood vessels and lymphatics. Methods in Enzymology 445, 27-52, 2008.

154. Mehta S.R., Huang, R., Yang, M., Zhang, X-Q., Kolli, B., Chang, K-P., Hoffman, R.M., Goto, Y., Badaro, R., and Schooley, R.T. Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery. Clinical and Vaccine Immunology 15, 1764-1770, 2008.

155. Amoh, Y., Li, L., Katsuoka, K., and Hoffman, R.M.  Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function.  Cell Cycle 7, 1865-1869, 2008. News: Supplemental Movie 1- Before treatment: Supplemental Movie 2- After treatment:

156. Amoh, Y., Katsuoka, K., and Hoffman, R.M. Color-coded fluorescent protein imaging of tumor angiogenesis: the AngioMouse models. Curr. Pharm. Des. 14, 3810-3819, 2008.

157. McElroy, M., Hayashi, K., Garmy-Susini, B., Kaushal, S., Varner, J.A., Moossa, A.R., Hoffman, R.M., and Bouvet, M. Fluorescent LYVE-1 antibody to image dynamically lymphatic trafficking of cancer cells in vivo. Journal of Surgical Research 151, 68-73, 2009.

158. Amoh, Y., Kanoh, M., Niiyama, S., Kawahara, K., Satoh, Y., Katsuoka, K., and Hoffman, R.M. Human and mouse hair follicles contain separate niches for multipotent and monopotent stem cells. Cell Cycle 8, 176-177, 2009.

159. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M. A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induce angiogenesis and neurogenesis. Cell Proliferation 42, 75-82, 2009. [Please contact the AntiCancer office for a PDF copy]

160. Hoffman, R.M. Comment re: Development of a preclinical model of spontaneous melanoma metastasis. Cancer Research 69, 719, 2009.

161. Yang, M., Reynoso, J., Bouvet, M., and Hoffman, R.M. A transgenic red fluorescent protein-expressing nude mouse for color-coded imaging of the tumor microenvironment. J. Cell. Biochem. 106, 279-284, 2009.

162. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., Yamashita, S. In vitro/in vivo biorecognition of lectin-immobilized fluorescent nanospheres for human colorectal cancer cells. Journal of Controlled Release 134, 2-10, 2009.

163. Tran Cao, H.S., Kimura, H., Kaushal, S., Snyder, C.S., Reynoso, J., Hoffman, R.M., and Bouvet, M. The cyan fluorescent protein (CFP) transgenic mouse as a model for imaging pancreatic exocrine cells. Journal of the Pancreas 10, 152-156, 2009.

164. Hoffman, R.M. Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins. Clin. Exp. Metastasis 26, 345-355, 2009.

165. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. Cell Cycle 8, 870-875, 2009. News & Views

166. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., and Hoffman, R.M. Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhymurium. J. Cell. Biochem. 106, 992-998, 2009.

167. Andresen, V., Alexander, S., Heupel, W-M., Hirschberg, M., Hoffman, R.M., and Friedl, P. Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging. Current Opinion in Biotechnology 20, 2009, Epub ahead of print. DOI: 10.1016/j.copbio.2009.02.008.

168. Tran Cao, H.S., Reynoso, J., Yang M., Kimura, H., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet M. Development of the transgenic cyan fluorescent protein (CFP)-expressing nude mouse for “Technicolor” cancer imaging. J. Cell. Biochem 107, 328-334, 2009.

169. Snyder, C.S., Kaushal, S., Kono, Y., Tran Cao, H.S., Hoffman, R.M., and Bouvet, M. Complementarity of ultrasound and fluorescence imaging in an orthotopic mouse model of pancreatic cancer. BMC Cancer 9, 106, 2009. DOI: 10.1117/1.3103783.

170. Matsuo, Y., Raimondo, M., Woodward, T.A., Wallace, M.B., Gill, K.R., Tong, Z., Burdick, M.D., Yang, Z., Strieter, R.M., Hoffman, R.M., Guha, S. CXC-chemokine/CXCR2 biological axis promotes angiogenesis in vitro and in vivo in pancreatic cancer. Int. J. Cancer, in press, 2009.

171. Nagakura, C., Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice. Anticancer Res. 29, 1873-1878, 2009.

172. McElroy, M., Kaushal, S., Tran Cao, H.S., Moossa, A.R., Talamini, M.A., Hoffman, R.M., Bouvet, M. Upregulation of thrombospondin-1 and angiogenesis in an aggressive human pancreatic cancer cell line selected for high metastasis. Molecular Cancer Therapeutics 8, 1779-1786, 2009.

173. Ma, H., Das, T., Pereira, S., Yang, Z., Zhao, M., Mukerji, P., and Hoffman, R.M. Efficacy of dietary antioxidants combined with a chemotherapeutic agent on human colon cancer progression in a fluorescent orthotopic mouse model. Anticancer Research 29, 2421-2426, 2009.

174. Hoffman, R.M. Dual-color imaging of tumor angiogenesis. In: Methods in Molecular Biology, Vol. 515, Viral Applications of Green Fluorescent Protein, pp. 45-61. Hicks, Barry W., ed. New York, NY: Humana Press, 2009.

175.  Makale, M., McElroy, M., O’Brien, P., Hoffman, R.M., Guo, S., Bouvet, M., Barnes, L., Ingulli, E., and Cheresh, D.  Extended-working-distance multiphoton micromanipulation microscope for deep-penetration imaging in live mice and tissue.  Journal of Biomedical Optics 14(2), 024032, 2009.

176. Kishimoto, H., Zhao, M., Hayashi, K., Urata, Y., Tanaka, N., Fujiwara, T., Penman, S., and Hoffman, R.M. In vivo internal tumor illumination by telomerase-dependent adenoviral GFP for precise surgical navigation. Proc. Natl. Acad. Sci. USA, 1-6, 14514-14517, 2009. Highlights (please see Medical Sciences):  Science Magazine Editor’s Choice:   MIT Technology Review: BioPhotonics:

177. Folkins, C., Shaked, Y., Man, S., Tang, T., Lee, C.R., Zhu, Z., Hoffman, R.M., and Kerbel, R.S. Glioma tumor stem-like cells promote tumor angiogenesis and vasculogenesis via vascular endothelial growth factor and stromal-derived factor 1. Cancer Research 69, 7243-7251, 2009.

178. Zhou, J., Yu, Z., Zhao, S., Hu, L., Zheng, J., Yang, D., Bouvet, M., Hoffman, R.M. Lentivirus-based DsRed-2 transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease.  J. Surg. Res. 157, 63-70, 2009.

179. Tome, Y., Tsuchiya, H., Hayashi, K., Yamauchi, K., Sugimoto, N., Kanaya, F., Tomita, K., and Hoffman, R.M. In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. J. Cell. Biochem. 108, 362-367, 2009. DOI: 10.1002/jcb.22259.

180. Penman, S., Hoffman, R.M. Mouse liver is shown with GFP. Science, E-Letter Response, August 17, 2009.

         Response to: Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY. Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Science 324, 804-807, 2009.

181. Daenen, L.G., Shaked, Y., Man, S., Xu, P., Voest, E.E., Hoffman, R.M., and Kerbel, R.S. Comparison of the efficacy of combining metronomic chemotherapy with a vascular disrupting agent (VDA) on primary versus advanced metastatic cancer. Molecular Cancer Therapeutics 8, 2872-2881, 2009.

182. Kishimoto, H., Urata, Y., Tanaka, N., Fujiwara, T., and Hoffman, R.M. Selective metastatic tumor labeling with green fluorescent protein and killing by systemic administration of telomerase-dependent adenoviruses. Molecular Cancer Therapeutics 8, 3001-3008, 2009. 

183. Bouvet, M., and Hoffman, R.M. Clinically-relevant orthotopic metastatic models of pancreatic cancer imageable with fluorescent genetic reporters. Minerva Chirurgica 64, 521-539, 2009.

184. Kimura, H., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Real-time imaging of single cancer-cell dynamics of lung metastasis. J. Cell. Biochem. 109, 58-64, 2010.

185. Hasegawa, A., Hayashi, K., Kishimoto, H., Yang, M., Tofukuji, S., Suzuki, K., Nakajima, H., Hoffman, R.M., Shirai, M., and Nakayama, T. Color-coded real-time cellular imaging of lung T lymphocyte accumulation and focus formation in a mouse asthma model. J. Allergy Clin. Immunol 125, 461-468, 2010.

186. Murphy, E.A., Shields, D.J., Stoletov, K., Dneprovskaia, E., McElroy, M., Greenberg, J.I., Lindquist, J., Acevedo, L.M., Anand, S., Majeti, B.K., Tsigelny, I., Saldanha, A., Walsh, B., Hoffman, R.M., Bouvet, M., Klemke, R.L., Vogt, P.K., Arnold, L., Wrasidlo, W., and Cheresh, D.A. Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFRβ/B-RAF. Proc. Natl. Acad. Sci. USA 107, 4299-4304, 2010.

187. Logan-Collins, J., Thomas, R.M., Yu, P., Jaquish, D., Mose, E., French, R., Stuart, W., McClaine, R., Aronow, B., Hoffman, R.M., Waltz, S.E., and Lowy, A.M. Silencing of RON receptor signaling promotes apoptosis and gemcitabine sensitivity in pancreatic cancers. Cancer Res. 70, 1130-40, 2010.

188. Kioi, M., Vogel, H., Schultz, G., Hoffman, R.M., Harsh, G.R., and Brown, J.M. Inhibition of vasculogenesis, but not angiogenesis, prevents the recurrence of glioblastoma following irradiation in mice. J Clin Invest 120, 694-705, 2010.

189. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K.I., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., and Yamashtia, S. Detection of early colorectal cancer imaged with peanut agglutinin-immobilized fluorescent nanospheres having surface poly(N-vinylacetamide) chains. Eur. J. Pharma. Biopharma. 74, 451-460, 2010.

190. Kimura, H., Zhang, L., Zhao, M., Hayashi, K., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M. Targeted therapy of spinal cord glioma with a genetically-modified Salmonella typhimurium. Cell Proliferation 43, 41-48, 2010.

191. Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R. Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo. PLoS One 5, e9712, 2010.

192. Ishihara, Y., Matsunaga, K., Iijima, H., Hasegawa, G., Suzuki, T., Sato, A., Kobayashi, T., Yang, M., and Hoffman, R.M. The combination of 5-FU, leucovorin and CPT-11 (FOLFIRI) prolongs survival through inhibition of metastasis in an orthotopic model of colon cancer. Anticancer Res. 30, 403-408, 2010.

193. Zhang, Q., Yang, M., Shen, J., Gerhold, L.M., Hoffman, R.M., Xing, H.R. The role of the intravascular microenvironment in spontaneous metastasis development. International Journal of Cancer 126, 2534-2541, 2010.

194. Gros, S.J., Dohrmann, T., Peldschus, K., Schurr, P.G., Kaifi, J.T., Kalinina, T., Reichelt, U., Mann, O., Strate, T.G., Adam, G., Hoffman, R.M., and Izbicki, J.R. Complementary use of fluorescence and magnetic resonance imaging of metastatic esophageal cancer in a novel orthotopic mouse model. International Journal of Cancer 126, 2671-2681, 2010.

195. Mack, G., Marshall, A. Lost in migration. Nature Biotechnology 28, 214-229, 2010 (See page 218, Box 3).  

196. Zhang, Q., Yang, M., Shen, J., Gerhold, L.M., Hoffman, R.M., Xing, H.R. The role of the intravascular microenvironment in spontaneous metastasis development. International Journal of Cancer 126, 2534-2541, 2010.

197. Overton, G. Molecules can't hide from the newest in-vivo imaging techniques. Laser Focus World 46, May 2010.

198. Gros, S., Kurschat, N., Dohrmann, T., Reichelt, U., Dancau, A-M., Peldschus, K., Adam, G., Hoffman, R.M., Izbicki, J.R., Kaifi, J.T. Therapeutic targeting of the HER-2 receptor in a highly metastatic orthotopic model of esophageal carcinoma. Molecular Cancer Therapeutics 9, 2037-2045, 2010.

199. Tran Cao, H.S., Bouvet, M., Kaushal, S., Keleman, A., Romney, E., Kim, G., Fruehauf, J., Imagawa, D.K., Hoffman, R.M., Katz, M. Low-dose metronomic gemcitabine in combination with Sunitinib inhibits multi-site metastasis and increases survival in an orthotopic model of pancreatic cancer. Molecular Cancer Therapeutics 9, 2068-2078, 2010. (Highlights Section. Molecular Cancer Therapeutics 9, 1929, 2010 ).

200. Wessels, J.T., Yamauchi, K., Hoffman, R.M., and Wouters, F.S. Advances in cellular, subcellular, and nanoscale imaging in vitro and in vivo. Cytometry A 77, 667-676, 2010.

201. Kimura, H., Lee, C., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo. J. Cell. Biochem 110, 1439-1446, 2010.

202. Tsai, M-H., Aki, R., Amoh, Y., Hoffman, R.M., Katsuoka, K., Kimura, H., Lee, C., and Chang, C-H. GFP-fluorescence-guided UVC irradiation inhibits melanoma growth and angiogenesis in nude mice. Anticancer Research 30, 3291-3294, 2010.

203. Gros, S.J., Dohrmann, T., Kaifi, J.T., Bouvet, M., Hoffman, R.M., Izbicki, J.R. Orthotopic fluorescent peritoneal carcinomatosis model of esophageal cancer. Anticancer Research 30, 3933-3938, 2010.

204. Suetsugu, S., Osawa, Y., Nagaki, M., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M. Simultaneous color-coded imaging to distinguish cancer “stem-like” and non-stem cells in the same tumor. J. Cell. Biochem. 111, 1035-1041, 2010.

204. Tran Cao, H.S., Kaushal, S., Snyder, C.S., Ongkeko, W., Hoffman, R.M., and Bouvet, M. Real-time imaging of tumor progression in a fluorescent orthotopic mouse model of thyroid cancer. Anticancer Research 30, 4415-4422, 2010.

205. Erten, A., Hall, D., Hoh, C., Tran Cao, H.S., Kaushal, S., Esener, S., Hoffman, R.M., Bouvet, M., Chen, J., Kesari, S., and Makale, M. Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging. J. Biomed. Optics 15(6), 066012, 2010.

206. Hoffman, R.M. Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer. In: Light Microscopy. Methods in Molecular Biology 689. H. Chiarini-Garcia, R.C.N. Melo, eds. Springer Science+Business Media, LLC, 183-204, 2011. DOI: 10.1007/978-1-60761-950-5.

207. Tran Cao, H., Kaushal, S., Lee, C., Snyder, C.S., Thompson, K.J., Horgan, S., Talamini, M.A., Hoffman, R.M., Bouvet, M. Fluorescence laparoscopy imaging of pancreatic tumor progression in an orthotopic mouse model. Surg. Endo. 25, 48-52, 2011.

208. Bobek, V., Kolostova, K., Pinterova, D., Kacprzak, G., Adamiak, J., Kolodzej, J., Boubelik, M., Kubecova, M., and Hoffman, R.M. A clinically-relevant, syngeneic model of spontaneous, high-metastatic B16 mouse melanoma. Anticancer Research 30, 4799-4804, 2010.

209. Hoffman, R. Orthotopic mouse models expressing fluorescent proteins for cancer drug discovery. Expert Opin Drug Discov. 5, 851-866, 2010.

210. Suetsugu, A., Osawa, Y., Nagaki, M., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Imaging the recruitment of cancer-associated fibroblasts by liver-metastatic colon cancer. J. Cell. Biochem. 112, 949-953, 2011.

211. Ilina, O., Bakker, G-J., Vasaturo, A., Hoffman, R.M., and Friedl, P. Two-photon laser-generated microtracks in 3D collagen lattices: principles of MMP-dependent and -independent collective cancer cell invasion. Physical Biology 8, 015010 (8 pp) 2011.

212. Tan, W., Zhang, W., Strasner, A., Grivennikov, S., Cheng, J.Q., Hoffman, R.M., and Karin, M. Tumour-infiltrating regulatory T cells stimulate mammary cancer metastasis through RANKL-RANK signalling. Nature 470, 548-553, 2011.

213. Liu, A., Fong, A., Becket, E., Yuan, J., Tamae, C., Medrano, L., Maiz, M., Wahba, C., Lee, C., Lee, K., Tran, K.P., Yang, H., Hoffman, R.M., Salih, A., and Miller, J.H. Selective advantage of resistant strains at trace levels of antibiotics: a simple and ultrasensitive color test for detection of antibiotics and genotoxic agents. Antimicrobial Agents and Chemotherapy 55, 1204-1210, 2011.

214. Soda, Y., Marumoto, T., Friedmann-Morvinski, D., Soda, M., Liu, F., Michiue, H., Pastorino, S., Yang, M., Hoffman, R.M., Kesari, S., Verma, I.M. Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc. Natl. Acad. Sci. USA 108, 4274-4280, 2011.
Commentary: Hormigo, A., Ding, B-S., and Rafii, S. A target for antiangiogenic therapy: Vascular endothelium derived from glioblastoma. Proc. Natl. Acad. Sci. USA 108, 4271-4272, 2011.

215. Bhattacharya, A., Turowski, S.G., San Martin, I.D., Rajput, A., Rustum, Y.M., Hoffman, R.M., and Seshadri, M. Magnetic resonance and fluorescence-protein imaging of the anti-angiogenic and anti-tumor efficacy of selenium in an orthotopic model of human colon cancer. Anticancer Research 31, 387-394, 2011. 

216. Hoffman, R.M. Imaging the steps of metastasis at the macro and cellular level with fluorescent proteins in real time (Chapter 6). In: Tumor Models in Cancer Research, vol. 2. Teicher, B.A., ed. Humana Press (c/o Springer Science+Business Media LLC), 125-166, 2011.  

217. Kolostova, K., Pinterova, D., Hoffman, R.M., and Bobek, V. Circulating human prostate cancer cells from an orthotopic mouse model rapidly captured by immunomagnetic beads and imaged by GFP expression. Anticancer Res. 31, 1535-1539, 2011.  

218. Zhang, Q., Bindokas, V.P., Shen, J., Fan, H., Hoffman, R.M., Xing, H.R. Time-course imaging of therapeutic functional tumor vascular normalization by anti-angiogenic agents. Mol. Cancer Ther. 10, 1173-1184, 2011.  

219. Tran Cao, H.S., Kaushal, S., Menen, R.S., Metildi, C.A., Lee, C., Snyder, C.S., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Submillimeter-resolution fluorescence laparoscopy of pancreatic cancer in an orthotopic mouse model visualizes metastases not seen with standard laparoscopy. J. Laparoendosc. Adv. Surg. Tech. A 21, 485-489, 2011.  

220. Kishimoto, H., Aki, R., Urata, Y., Bouvet, M., Momiyama, M., Tanaka, N., Fujiwara, T., and Hoffman, R.M. Tumor-selective adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection. Cell Cycle 10, 2737-2741, 2011.  

221. Abou-Elkacem, L., Gremse, F., Barth, S., Hoffman, R.M., Kiessling, F., and Lederle, W. Comparison of µCT, MRI and optical reflectance imaging for assessing the growth of GFP/RFP-expressing tumors. Anticancer Res. 31, 2907-2913, 2011.  

222. Menen, R.S., Pinney, E., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M. A rapid imageable in vivo metastasis assay for circulating tumor cells. Anticancer Res. 31, 3125-3128, 2011. 

223. Grzesiak, J.J., Tran Cao, H.S., Burton, D.W., Kaushal, S., Vargas, F., Clopton, P., Snyder, C.S., Deftos, L.J., Hoffman, R.M., and Bouvet, M. Knockdown of the β1 integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis. Int. J. Cancer 129, 2905-2915, 2011. 

224. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., Gurlich, R., and Hoffman, R.M. Tail spontaneous metastatic mouse model: Comparison of metastatic potential of orthotopic and heterotopic models imaged by GFP and RFP protein. In Vivo 25, 849-852, 2011. 

225. Hassanein, M.K., Suetsugu, A., Saji, S., Moriwaki, H., Bouvet, M., Moossa, A.R., and Hoffman, R.M. Stem-like and non-stem human pancreatic cancer cells distinguished by morphology and metastatic behavior. Journal of Cellular Biochemistry 112, 3549-3554, 2011 (Cover story). 

226. Murata, T., Mizushima, H., Chinen, I., Moribe, H., Yagi, S., Hoffman, R.M., Kimura, T., Yoshino, K., Ueda, Y., Enomoto, T., and Mekada, E. HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts to support progression of uterine cervical cancers. Cancer Res. 71, 6633-6642, 2011. 

227. Bouvet, M., and Hoffman, R.M. Glowing tumors make for better detection and resection. Sci. Transl. Med. 3, 110sf10, 2011. 

228. Hayashi, K., Kimura, H., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Hasegawa, A., Bouvet, M., and Hoffman, R.M. Comparison of cancer cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse. Anticancer Res. 31, 3665-3672, 2011. 

229. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., and Hoffman, R.M. Development of new spontaneous metastatic heterotopic model of Lewis lung carcinoma imaged by GFP Expression. Cancer Invest. 29, 692-695, 2011. 

230. Sakuma, S., Yamashita, S., Hiwatari, K., Hoffman, R.M., and Pham, W. Lectin-immobilized fluorescent nanospheres for targeting to colorectal cancer from a physicochemical perspective. Curr. Drug Discov. Technol. 8, 367-378, 2011. 

231. Suetsugu, A., Hassanein, M.K., Reynoso, J., Osawa, Y., Nagaki, M., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M. The cyan fluorescent protein nude mouse as a host for multicolor-coded imaging models of primary and metastatic tumor microenvironments. Anticancer Res. 32, 31-38, 2012. 

232. Yamauchi, K., Tome, Y., Yamamoto, N., Hayashi, K., Kimura, H., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman R.M. Color-coded real-time subcellular fluorescence imaging of the interaction between cancer and host cells in live mice. Anticancer Res. 32, 39-44, 2012. 

233. Amoh, Y., Hamada, Y., Katsuoka, K., Hoffman, R.M. In vivo imaging of nuclear-cytoplasmic deformation and partition during cancer cell death due to immune reaction. J. Cell. Biochem. 113, 465-472, 2012 (Cover story)

234. Bakker, G-J., Andresen, V., Hoffman, R.M., and Friedl, P. Fluorescence lifetime microscopy of tumor cell invasion, drug delivery, and cytotoxicity. In: Methods in Enzymology, Imaging and Spectroscopic Analysis of Living Cells: Optical and Spectroscopy Techniques. Vol. 504, 109-125. P.Michael Conn, ed. Academic Press: Burlington, 2012.

235. Hoffman, R.M. Live cell imaging in live animals with fluorescent proteins. In: Methods in Enzymology, Imaging and spectroscopic analysis of living cells. Imaging live cells in health and disease. Vol. 506, 197-204, P. Michael Conn, ed. Elsevier, 2012.

236. Hoffman, R.M. Cellular and subcellular imaging in live mice using fluorescent proteins. Current Pharmaceutical Biotechnology 13, 537-544, 2012.

237. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens. Anticancer Research 32, 1175-1180, 2012.

238. Yamazaki, T., Aoki, K., Heike, Y., Kim, S.W., Ochiya, T., Wakeda, T., Hoffman, R.M., Takaue, Y., Nakagama, H., and Ikarashi, Y. Real-time in vivo cellular imaging of graft-versus-host disease and its reaction to immunomodulatory reagents. Immunol. Lett. 144, 33-40, 2012. 

239. Wang, M., Zhang, X., Xiong, X., Yang, Z., Sun, Y., Yang, Z., Hoffman, R.M., and Liu, Y. Efficacy of the Chinese Traditional Medicinal Herb Celastrus orbiculatus Thunb on human hepatocellular carcinoma in an orthotopic fluorescent nude mouse model. Anticancer Res. 32, 1213-1220, 2012.

240 Kelber, J.A., Reno, T., Kaushal, S., Metildi, C., Wright, T., Stoletov, K., Weems, J.M., Park, F.D., Mose, E., Wang, Y., Hoffman, R.M., Lowy, A.M., Bouvet, M., and Klemke, R.L. KRas induces a Src/PEAK1/ErbB2 kinase amplification loop that drives metastatic growth and therapy resistance in pancreatic cancer. Cancer Research 72, 2554-2564,2012.

241 Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Moriwaki, H., Bouvet, M., Saji, S., and Hoffman, R.M. Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens. J. Cell. Biochem. 113, 2290-2295, 2012.

242 Duong, J., Mii, S., Uchugonova, A., Liu, F., Moossa, A.R., and Hoffman, R.M. Real-time confocal imaging of trafficking of nestin-expressing multipotent stem cells in mouse whiskers in long-term 3-D histoculture.  In Vitro Cell. Dev. Biol. Anim. 48, 301-305, 2012. 

243 Metildi, C.A., Kaushal, S., Lee, C., Hardamon, C.R., Snyder, C.S., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M.  An LED light source and novel fluorophore combinations improve fluorescence laparoscopic detection of metastatic pancreatic cancer in orthotopic mouse models.  J. Am. Coll. Surg. 214, 997-1007, 2012.

244 Menen, R., Pinney, E., Hassanein, M.K., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M.  Inhibition of metastasis of circulating human prostate cancer cells in the chick embryo by an extracellular matrix produced by foreskin fibroblasts in culture.  Anticancer Research 32, 1573-1577, 2012.

245 Hu, M., Zhao, M., An, C., Yang, M., Li, Q., Zhang, Y., Suetsugu, A., Tome, Y., Yano, S., Fu, Y., Hoffman, R.M., and Hu, K.  Real-time imaging of apoptosis induction of human breast cancer cells by the traditional Chinese medicinal herb Tubeimu.  Anticancer Res. 32, 2509-2514, 2012.

246 Bouvet, M., and Hoffman, R.M.  In vivo imaging of pancreatic cancer with fluorescent proteins in mouse models (Chap. 4).  In  In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols.  Hoffman, R.M., ed.  Methods in Molecular Biology, Vol. 872.  Walker, John M., series ed.  Humana Press (Springer Science+Business Media New York), 2012.

247 Yu, Z., Zhou, J., and Hoffman, R.M.  Lentivirus-based DsRed-2-transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease (Chap. 5).  In: In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols.  Hoffman, R.M., ed.  Methods in Molecular Biology, Vol. 872.  Walker, John M., series ed.  Humana Press (Springer Science+Business Media New York), 2012.

248 Hoffman, R.M.  Subcellular imaging in vivo: the next GFP revolution (Chap. 18).  In: In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols.  Hoffman, R.M., ed.  Methods in Molecular Biology, Vol. 872.  Walker, John M., series ed.  Humana Press (Springer Science+Business Media New York), 2012.

249 Hoffman, R.M.  Bugging Tumors.  Cancer Discovery 2(7), 588-390, 2012.

250 Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M.  Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer-patient tumorgraft progression in nude mice. Anticancer Research 32, 3063-3068, 2012.

251 Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M.  Imageable Fluorescent Metastasis Resulting in Transgenic GFP Mice Orthotopically Implanted with Human-patient Primary Pancreatic Cancer Specimens. Anticancer Research 32, 1175-1180, 2012.

252 Amoh, Y., Mii, S., Aki, R., Hamada, Y., Kawahara, K., Hoffman, R.M., and Katsuoka, K.  Multipotent nestin-expressing stem cells capable of forming neurons are located in the upper, middle and lower part of the vibrissa hair follicle.  Cell Cycle 11, 3513-3517, 2012.

253 Kimura, H., Tome, Y., Momiyama, M., Hayashi, K., Tsuchiya, H., Bouvet, M., and Hoffman, R.M.  Imaging the inhibition by anti-b1 integrin antibody of lung seeding of single osteosarcoma cells in live mice.  Int. J. Cancer 131, 2027-2033, 2012.  DOI: 10.1002/ijc.27475.

254 Tran Cao, H.S., Kaushal, S., Metildi, C.A., Menen, R.S., Lee, C., Snyder, C.S., Messer, K., Pu, M., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M.  Tumor-specific fluorescence antibody imaging enables accurate staging laparoscopy in an orthotopic model of pancreatic cancer.  Hepatogastroenterology 59(118), 1994-1999, 2012.  DOI: 10.5754/hge11836.

255 Momiyama, M., Suetsugu, A., Kimura, H., Kishimoto, H., Aki, R., Yamada, A., Sakurada, H., Chishima, T., Bouvet, M., Bulgakova, N.N., Endo, I., and Hoffman, R.M.  Fluorescent proteins enhance UVC PDT of cancer cells.  Anticancer Research 32, 4327-4330, 2012.

256 Momiyama, M., Kumamoto, T., Suetsugu, A., Kishimoto, H., Chishima, T., Tanaka, K., Akiyama, H., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M.  Major liver resection stimulates stromal recruitment and metastasis compared with repeated minor resection.  J. Surg. Res. 178, 280-287, 2012.

257 Uchugonova, A., Zhao, M., Zhang, Y., Weinigel, M., König, K., and Hoffman, R.M.  Cancer-cell killing by engineered Salmonella imaged by multiphoton tomography in live mice.  Anticancer Research 32, 4331-4338, 2012.

258 Hoffman, R.M., and Bouvet, M. Shedding (killer) light on tumors. Semin. Thorac. Cardiovasc. Surg. 24, 235-237, 2012.

259 Metildi, C.A., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet, M.  Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model.  J. Surg. Res. 179, 87-93, 2013.

260 Uchugonova, A., Zhao, M., Weinigel, M., Zhang, Y., Bouvet, M., Hoffman, R.M., and Koenig, K.  Multiphoton tomography visualizes collagen fibers in the tumor microenvironment that maintain cancer-cell anchorage and shape.   J. Cell. Biochem. 114, 99-102, 2013.  DOI: 10.1002/jdb.24305.

261 Tome, Y., Zhang, Y., Momiyama, M., Maehara, H., Kanaya, F., Tomita, K., Tsuchiya, H., Bouvet, M., Hoffman, R.M.., and Zhao, M.  Primer dosing of S. typhimurium A1-R potentiates tumor-targeting and efficacy in immunocompetent mice.  Anticancer Res. 33, 97-102, 2013.

262 Momiyama, M., Suetsugu, A., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M.  Subcellular real-time imaging of the efficacy of temozolomide on cancer cells in the brain of live mice.  Anticancer Res. 33, 103-106, 2013.

263 Momiyama, M., Hiroshima, Y., Suetsugu, A., Tome, Y., Mii, S., Yano, S., Bouvet, M., Chishima, T., Endo, I., and Hoffman, R.M.  Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice.  Anticancer Res. 33, 107-112, 2013.

264 Momiyama, M., Suetsugu, A., Kimura, H., Kishimoto, H., Aki, R., Yamada, A., Sakurada, H., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M.  Imaging the efficacy of UVC irradiation on superficial brain tumors and metastasis in live mice at the subcellular level.  J. Cell. Biochem. 114, 428-434, 2013.

265 Hoffman, R.M.  Fluorescent proteins as visible in vivo sensors.  In:  Progress in Molecular Biology and Translational Science, Volume 113, pp. 87-93. Morris, M., ed.  Elsevier, 2013.

266 Shi, Y., Tong, M., Wu, Y., Yang, Z., Hoffman, R.M., Zhang, Y., Tian, Y., Qi, M., Lin, Y., Liu, Y., Dai, L., Sun, Y., and Wang, Z.  VEGF-C ShRNA inhibits pancreatic cancer growth and lymphangiogenesis in an orthotopic fluorescent nude mouse model.  Anticancer Research 33, 409-417, 2013.

267 Suetsugu, A., Honma, K., Saji, S., Moriwaki, H., Ochiya, T., and Hoffman, R.M. Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude mouse models. Adv. Drug Deliv. Rev. 65, 383-390, 2013.

268 Miwa, S., Tome, Y., Yano, S., Hiroshima, Y., Uehara, F., Mii, S., Kimura, H., Hayashi, K., Tsuchiya, H., Bouvet, M., Efimova, E.V., and Hoffman, R.M. Single cell time-lapse imaging of focus formation by the DNA damage-response protein 53BP1 after UVC irradiation of human pancreatic cancer cells. Anticancer Res. 33, 1373-1378, 2013.

269 Momiyama, M., Suetsugu, A., Kimura, H., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M. Dynamic subcellular imaging of cancer cell mitosis in the brain of live mice. Anticancer Res. 33, 1367-1371, 2013.

270 Uehara, F., Tome, Y., Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Bouvet, M., Maehara, H., Kanaya, F., and Hoffman, R.M. A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res. 33, 1361-1366, 2013.

271 Metildi, C.A., Hoffman, R.M., and Bouvet, M. Fluorescence-guided surgery and fluorescence laporascopy for gastrointestinal cancers in clinically-relevant mouse models. Gastroenterol. Res. Pract. 2013, Article ID 290634, 8 pages, 2013.

272 Steinkamp, M.P., Winner, K.K., Davies, S., Muller, C., Zhang, Y., Hoffman, R.M., Shirinifard, A., Moses, M., Jiang, Y., and Wilson, B.S. Ovarian tumor attachment, invasion, and vascularization reflect unique microenvironments in the peritoneum: insights from xenograft and mathematical models. Frontiers in Oncology, published online, 2013. DOI: 10.3399/fonc.2013.00097.

273 Zhang, Y., Zhang, N., Su, S., Hoffman, R.M., Zhao, M. Salmonella typhimurium A1-R tumor targeting in immunocompetent mice is enhanced by a Traditional Chinese Medicine herbal mixture. Anticancer Res. 33, 1837-1843, 2013.

274 Hoffman, R.M. Stromal-cell and cancer-cell exosomes leading the metastatic exodus for the promised niche. Breast Cancer Res. 15, 310-313, 2013.

275 Wu, J., Ma, R., Cao, H., Wang, Z., Jing, C., Sun, Y., Zhang, Y., Yang, Z., Hoffman, R.M., and Tang, J. Intraoperative imaging of metastatic lymph nodes using a fluorophore-conjugated antibody in a HER2/neu-expressing orthotopic breast cancer mouse model. Anticancer Research 33, 419-424, 2013.

276 Rashidi, B., Moossa, A.R., and Hoffman, R.M. Specific route mapping visualized with GFP of single-file streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J. Cell. Biochem 114, 1738-1743, 2013.

277 Wolf, K., te Lindert, M., Krause, M., Alexander, S., te Riet, J., Willis, A.L., Hoffman, R.M., Figdor, C.G., Weiss, S.J., and Friedl, P. Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force. J. Cell. Biol. 201, 1069-1084, 2013.

278 Snyder, C.S., Harrington, A.R., Kaushal, S., Mose, E., Lowy, A.M., Hoffman, R.M., and Bouvet, M. A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment. Pancreas 42, 952-958, 2013.

279 Zhang, L., Wu, C., Zhang, Y., Liu, F., Zhao, M., Bouvet, M., and Hoffman, R.M. Efficacy comparison of traditional Chinese medicine LQ versus gemcitabine in a mouse model of pancreatic cancer. J. Cell. Biochem. 114, 2131-2137, 2013.

280 Hoffman, R.M. Cell Markers: Green Fluorescent Protein. In: Brenner’s Encyclopedia of Genetics, 2nd Ed., Vol. 7, pp. 483-487. Elsevier, 2013.

281 Uehara, F., Tome, Y., Reynoso, J., Mii, S., Yano, S., Miwa, S., Bouvet, M., Maehara, H., Kanaya, F., Moossa, A.R., and Hoffman, R.M. Color-coded imaging of spontaneous vessel anastomosis in vivo. Anticancer Research 33, 3041-3045.

282 Tome, Y., Sugimoto, N., Yano, S., Momiyama, M., Mii, S., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Research 33, 3021-3025.

283 Ke, C-C., Liu, R-S., Suetsugu, A., Kimura, H., Ho, J.H., Lee, O.K., and Hoffman, R.M. In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells. PLoS One 8, e69658, 2013.

284 Hiroshima, Y., Zhao, M., Zhang, Y., Maawy, A., Hassanein, M.K., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Suetsugu, A., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. Cell Cycle 12, 2774-2780, 2013.

285 Tome, Y., Kimura, H., Maehara, H., Sugimoto, N., Bouvet, M., Tsuchiya, H., Kanaya, F., Hoffman, R.M. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of αvβ3 integrin. Anticancer Research 33, 3623-3628, 2013.

286 Metildi, C.A., Kaushal, S., Hoffman, R.M., and Bouvet, M. In vivo serial selection of human pancreatic cancer cells in orthotopic mouse models produces high metastatic variants irrespective of Kras status. J. Surg. Res. 184, 290-298, 2013.

287 Miwa, S., Yano, S., Tome, Y., Sugimoto, N., Hiroshima, Y., Uehara, F., Mii, S., Kimura, H., Hayashi, K., Efimova, E.V., Fujiwara, T., Tsuchiya, H., and Hoffman, R.M. Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy. J. Cell. Biochem. 114, 2454-2460, 2013.

288 Miwa, S., Yano, S., Hiroshima, Y., Tome, Y., Uehara, F., Mii, S., Efimove, E.V., Kimura, H., Hayashi, K., Tsuchiya, H., Hoffman, R.M. Imaging UVC-induced DNA damage response in models of minimal cancer. J. Cell. Biochem. 114, 2493-2499, 2013.

289 Suetsugu, A., Jiang, P., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M. Imaging nuclear-cytoplasm dynamics of cancer cells in the intravascular niche of live mice. Anticancer Research 33, 4229-4236, 2013.

290 He, G., Dhar, D., Nakagawa, H., Font-Burgada, J., Ogata, H., Jiang, Y., Shalapour, S., Seki, E., Yost, S.E., Jepsen, K., Frazer, K.A., Harismendy, O., Hatziapostolou, M.,Iliopoulos, D., Suetsugu, A., Hoffman, R.M., Tateishi, R., Koike, K., Karin, M. Identification of Liver Cancer Progenitors Whose Malignant Progression Depends on Autocrine IL-6 Signaling. Cell 155, 384-396, 2013.

291 Bouvet, M., and Hoffman, R.M. Laparoscopic fluorescence imaging for identification and resection of pancreatic and hepatobiliary cancer. Frontiers of Gastrointestinal Research, “Fluorescent Imaging in the Treatment of Hepatobiliary and Pancreatic Diseases: Techniques and Future Perspectives,” Kokudo, N., and Ishizawa, T., eds., Basel, Karger, 31, 92-99, 2013.

292 Hoffman, R.M. Imageable clinically-relevant mouse models of cancer. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 141-170. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.

293 Hoffman, R.M. Imaging metastatic cell trafficking at the cellular level in vivo with fluorescent proteins. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 171-179. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.