1.       Freeman, A., and Hoffman, R.M.  In vivo-like growth of human tumors in vitro.  Proc. Natl. Acad. Sci. USA 83, 2694-2698, 1986. [PDF]

2.     Vescio, R.A., Redfern, C.H., Nelson, T.J., Ugoretz, S., Stern, P.H., and Hoffman, R.M.  In vivo-like drug response of human tumors growing in three-dimensional, gel-supported, primary culture.  Proc. Natl. Acad. Sci. USA 84, 5029-5033, 1987. [PDF]

3.   Hoffman, R.M., Monosov, A.Z., Connors, K.M., Herrera, H., and Price, J.H.  A general native-state method for determination of proliferation capacity of human normal and tumor tissues in vitro.  Proc. Natl. Acad. Sci. USA 86, 2013-2017, 1989. [PDF]

4.     Wallen, J.W., Cate, R.L., Kiefer, D.M., Riemen, M.W., Martinez, D., Hoffman, R.M., Donahoe, P.K., Von Hoff, D.D., Pepinsky, B., and A. Oliff.  Minimal antiproliferative effect of recombinant Mullerian Inhibiting Substance on gynecological tumor cell lines and tumor explants.  Cancer Res. 49, 2005-2011, 1989. [PDF]

5.     Vescio, R.A., Connors, K.M., Youngkin, T., Bordin, G.M., Robb, J.A., Umbreit, J.N., and Hoffman, R.M.  Cancer biology for individualized cancer therapy: Correlation of growth fraction index in native-state culture with tumor grade and stage.  Proc. Natl. Acad. Sci. USA 87, 691-695, 1990. [PDF]

6.     Vescio, R.A., Connors, K.M., Bordin, G.M., Robb, J.A., Youngkin, T., Umbreit, J.N., and Hoffman, R.M.  The distinction of small cell and non-small cell cancer by growth in native-state histoculture.  Cancer Res. 50, 6095-6099, 1990. [PDF]

7.    Robbins, K.T., Varki, N.M., Storniolo, A.M., Hoffman, H., and Hoffman, R.M.  Drug response of head and neck tumors in native-state histoculture.  Archives of Otolaryngology Head and Neck Surg. 117, 83-86, 1991. [PDF]

8.   Hoffman, R.M.  Three-dimensional gel-supported native-state histoculture for evaluation of tumor-specific pharmacological activity: Principles, practices and possibilities.  J. Cell. Pharmacol. 2, 189-201, 1991. [PDF]

9.        Li, L., Hoffman, R.M.  Eye tissues grown in 3-dimensional histoculture for toxicological studies.  J. Cell.  Pharmacol. 2, 311-316, 1991. [PDF]

10.     Hoffman, R.M.  Three-dimensional histoculture: Origins and applications in cancer research.  Cancer Cells 3, 86-92, 1991. [PDF]

11.     Hoffman, R.M.  In vitro sensitivity assays in cancer: A review, analysis and prognosis.  J. Clin. Lab. Anal. 5, 133-143, 1991. [PDF]

12.    Vescio, R.A., Connors, K.M., Kubota, T., and Hoffman, R.M.  Correlation of histology and drug response of human tumors grown in native-state three-dimensional histoculture and in nude mice. Proc. Natl. Acad. Sci. USA 88, 5163-5166, 1991. [PDF]

13.    Guadagni, F., Roselli, M., and Hoffman, R.M.  Maintenance of expression of tumor antigens in three-dimensional in vitro human tumor gel-supported histoculture.  Anticancer Res. 11, 543-546, 1991. [PDF]

14.  Baibakov, B., Frank, G.A., Sergeeve, N., Youngkin, T., Connors, K.M., Hoffman, R.M., and Margolis, L.B.  In vivo growth patterns of human lung tumors in three-dimensional histoculture.  In Vitro Cell. Dev. Biol. 27A, 897-899, 1991. [PDF]

15. Furukawa, T., Kubota, T., Watanabe, M., Takahara, T., Yamaguchi, H., Takeuchi, T., Kase, S., Kodaira, S., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  High in vitro-in vivo correlation of drug response using sponge-gel-supported three-dimensional histoculture and the MTT end point.  Int. J. Cancer 51, 489-498, 1992. [PDF]

16.  Guo, H.Y., Colangelo, D., Li, L., Connors, K.M., Kubota, T., and Hoffman, R.M.  In vitro histoculture of human tumors with fluorescent dye end-points measured by confocal microscopy: high correlation of in vitro and in vivo chemosensitivity.  Anticancer Research 12, 1055-1062, 1992. [PDF]

17.  Guadagni, F., Li, L., and Hoffman, R.M.  Targeting antibodies to live tumor tissue in 3-D histoculture.  In Vitro Cell. Dev. Biol. 28A, 297-299, 1992. [PDF]

18.  Colangelo, D, Guo, H-Y, Silvestro, L., and Hoffman, R.M.  Noncolorimetric measurement of cell activity in three-dimensional histoculture using the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide: the pixel image analysis of formazan crystals.  Anal. Biochem. 205, 8-13, 1992. [PDF]

19.  Slocum, H.K., Toth, K. Li, L., Chang, S.G, Hoffman, R.M., and Rustum, Y.M.  Long-term passage of human tissues in vitro as three-dimensional histolines.  In Vitro Cell. Dev. Biol. 28A, 573-577, 1992. [PDF]

20.  Colangelo, D., Guo, Y.H., Connors, K.M., Kubota, T., Silvestro, L., and Hoffman, R.M.  Correlation of drug response in human tumors histocultured in vitro with an image-analysis MTT end point and in vivo xenografted in nude mice. Anticancer Res. 12, 1373-1376, 1992. [PDF]

21.  Geller, J., Sionit, L.R., Connors, K.M., and Hoffman, R.M.  Measurement of androgen sensitivity in the human prostate in in vitro three-dimensional histoculture.  The Prostate 21, 269-278, 1992. [PDF]

22.   Chang, S.G., Slocum, H., Toth, K., Hoffman, R.M., Perrapato, S.D., Huben, R.P., and Rustum, Y.M.  Glucose consumption end point in primary histoculture indicates recovery of human tumors from drug treatment.  In Vitro Cell. Dev. Biol. 28A, 585-587, 1992. [PDF]

23. Hoffman, R.M.  Histoculture and the immunodeficient mouse come to the cancer clinic: Rational approaches to individualizing cancer therapy and new drug evaluation (Review).  International Journal of Oncology 1, 467-474, 1992. [PDF]

24.  Hoffman, R.M.  To do tissue culture in two or three dimensions?  That is the question.  Stem Cells 11, 105-111, 1993. [PDF]

25.   Theodorescu, D., Connors, K.M., Groce, A., Hoffman, R.M., and Kerbel, R.S.   Lack of influence of c-Ha-ras expression on the drug sensitivity of human bladder cancer histocultured in three dimensions.  Anticancer Res. 13, 941-946, 1993. [PDF]

26.    Hoffman, R.M.  In vitro assays for chemotherapy sensitivity.   Crit. Rev. in Onc/Hem. 15, 99-111, 1993. [PDF]

27.    Geller, J., Sionit, L.R., Connors, K.M., Youngkin, T., and Hoffman, R.M.  Expression of prostate-specific antigen in human prostate specimens in in vitro three dimensional histoculture.  In Vitro Cell. Dev. Biol. 29A, 523-524, 1993. [PDF]

28.  Chang, S.G., Chai, S.O., Kim, E.S, Yoon, C., Joo, H.Z., and Hoffman, R.M.  The measurement of glucose consumption in histoculture to determine effects of doxorubicin and cisplatinum on human gastric carcinoma. Anticancer Res. 13, 1303-1310, 1993.  [PDF]

29.   Tanino, H., Kubota T., Saikawa, Y., Kuo, T-H., Takeuchi, T., Kase, S., Kurukawa, T., Kitajima, M. Saurai, T., Naito, Y. and Hoffman, R.M.  Different chemo- and endocrino-sensitivity of MCF‑7 cells with or without estradiol supplement in vitro. Anticancer Res. 13, 1219-1222, 1993.[PDF] 

30.  Robbins, T., Connors, K.M., Storniolo, A.M., Hanchett, C., and Hoffman, R.M.  Sponge-gel supported histoculture drug-response assay for head and neck cancer: Correlations with clinical response to cisplatin.  Arch. Otol. Head & Neck Surg. 120, 288-292, 1994. [PDF]

31.  Chang, S.G., Lee, J.H., Hong, D.H., Lee, H.Y., Chai, S.E. and Hoffman, R.M.  Comparison of glucose-consumption and thymidine-incorporation endpoints on histocultured human superficial bladder tumor.  Anticancer Res. 14, 77-83, 1994. [PDF]

32.  Hoffman, R.M.  The three-dimensional question: can clinically relevant tumor drug resistance be measured in vitro? Cancer and Metastasis Reviews 13, 169-173, 1994. [PDF]

33.   Geller, J., Sionit, L.R., Baird, A., Kohls, M., Connors, K.M., and Hoffman, R.M.  In vivo and in vitro effects of androgen on FGF-2 concentrations in the human prostate.  The Prostate 25, 206-209, 1994. [PDF]

34.   Furukawa, T., Kubota, T., Hoffman, R.M.  Clinical applications of the histoculture drug response assay.  Clinical Cancer Research 1, 305-311, 1995. [PDF]

35.   Kubota, T., Sasano, N., Abe, O., Nakao, I., Kawamura, E., Saito, T., Endo, M., Kimura, K., Demura, H., Sasano, H., Nagura, H., Ogawa, N., and Hoffman, R.M.  Potential of the histoculture drug response assay to contribute to cancer patient survival.  Clinical Cancer Research 1, 1537-1543, 1995. [PDF]

36.   Hoffman, R.M.  In vitro drug response assays for entry into the rational era cancer chemotherapy.  Human Cell 8, Japan Human Cell Society, 131-148, 1995. [PDF]

37.    Hoffman, R.M.  Can in vitro drug response assays be clinically useful?  Problems and solutions.  Biotherapy 10, 822-842, 1996.

38.  Chang, S-G., Jung, J-C., Rho, Y-S., Huh, J-S., Kim, J-I., and Hoffman, R.M.  Efficacy of the platinum analog {Pt(cis-dach)(DPPE) - 2NO3} on histocultured human patient bladder tumors and cancer cell lines.  Anticancer Res. 16, 3423-3428, 1996. [PDF]

39.  Chang, S-G., Lee, S.J., Lee, S-J., Kim, J.I., Jung, J-C. and Hoffman, R.M.  Interleukin-6 production in primary histoculture by normal human kidney and renal tumors tissues.  Anticancer Res. 17, 113-116, 1997. [PDF]

40.  Geller, J., Partido, C., Sionit, L., Youngkin, T., Espanol, M., Tan, Y., and Hoffman, R.M.  Comparison of androgen-independent growth and androgen-dependent growth in BPH and cancer tissue from the same radical prostatectomies in sponge-gel matrix histoculture.  The Prostate 31, 250-254, 1997. [PDF]

41.    Hoffman, R.M.   Taking  chemotherapy  from random  to rational  with  the  histoculture drug response  assay.

         Jpn. J. Cancer Chemother. 24, 206-229, 1997. [PDF]

42.    Chishima, T., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Yang, M., Shimada, H., Moossa, A.R., and Hoffman, R.M. The use of histoculture and green fluorescent protein to visualize tumor cell host interaction.  In Vitro Cell. Dev. Biol. 33, 745-747, 1997. [PDF]

43.  Geller, J., Sionit, L., Partido, C., Li, L., Tan, X-Y., Youngkin, T., Nachtsheim, D., and Hoffman, R.M.  Genistein inhibits the growth of human-patient BPH and prostate cancer in histoculture.  The Prostate 34, 75-79, 1998 [PDF]

44. Hoffman, R.M.  Three-dimensional sponge-gel matrix histoculture of human tumors:  Methods and applications. In: Cell Biology, A Laboratory Handbook 1, 367-379, Ed: J. Celis, San Diego: Academic Press, 1994. [PDF]

45. Hoffman, R.M.  Three-dimensional sponge-gel matrix histoculture of human tumors:  Methods and applications. In: Cell Biology, A Laboratory Handbook 2(1), 377-389, Ed: J. Celis, San Diego: Academic Press, 1998. [PDF]

46.   Olbina, G., Miljkovic, D., Hoffman, R.M., and Geller, J.  New sensitive discovery histoculture model for growth-inhibition studies in prostate cancer and BPH.  The Prostate 37, 126-129, 1998. [PDF]

47.    Haran, C.  Custom chemo.  MAMM, 49-53, September/October 1999. [PDF]

48.   Hoffman, R.M.  The clinical benefit of the histoculture drug response assay.  Jpn. J. Cancer Chemother 27, Supplement 11, 321-322, 2000. [PDF]

49.   Ohie, S., Udagawa, Y., Kozu, A., Komuro, Y., Aoki, D., Nozawa, S., Moossa, A.R., and Hoffman, R.M. Cisplatin sensitivity of ovarian cancer in the histoculture drug response assay correlates to clinical response to combination chemotherapy with cisplatin, doxorubicin, and cyclophosphamide. Anticancer Research 20, 2049-2054, 2000. [PDF]

50.   Furukawa, T., Kubota, T., Tanino, H., Oura, S., Yuasa, S., Murate, H., Morita, K., Kozakai, K., Yano, T., and Hoffman, R.M.  Chemosensitivity of breast cancer lymph node metastasis compared to the primary tumor from individual patients tested in the histoculture drug response assay.  Anticancer Research 20, 3657-3658, 2000. [PDF]

51.  Hoffman, R.M.  Histocultures and Organ Cultures. In: Encyclopedia of Life Sciences, http://www.els.net/, London: Nature Publishing Group, 2001. [PDF]

52. Tanino, H., Oura, S., Hoffman, R.M., Kubota, T., Furukawa, T., Arimoto, J., Yoshimasu, T., Hirai, I., Bessho, T., Suzuma, T., Sakuari, T., and Naito, Y. Acqusition of multidrug resistance in recurrent breast cancer demostrated by the histoculture drug response assay. Anticancer Res. 21, 4083-4086, 2001. [PDF]

53.  Singh, B., Li, R., Xu, L., Poluri, A., Patel, S., Shaha, A. S., Pfister, D., Sherman, E., Goberdhan, A., Hoffman, R. M., Shah, J.Prediction of survival in patients with head and neck cancer using the histoculture drug response assay. 24, Head&Neck. 437-442, 2002 [PDF]

54.  Flowers, JL., Hoffman, R.M., Driscoll, T.A., Wall, M.E., Wani, M.C., Manikumar, G., Friedman, H.S., Dewhirst, M., Colvin, O.M., Adams, D.J. The activity of camptothecin analogues is enhanced in histocultures of human tumors and human tumor xenografts by modulation of extracellular pH. Cancer Chemotherapy and Pharmacology 52, 253-261, 2003. [PDF]

55.   Hoffman, R.M.  Histocultures and their use.  In:  Encyclopedia of Life Sciences.  John Wiley and Sons, Ltd.: Chichester, 2010, Published Online.  DOI: 10.1002/9780470015902.a0002573.pub2. 

56.    Jung, P.S., Kim, D.Y., Kim, M.B., Lee, SW., Kim, J.H., Kim, Y.M., Kim, Y.T., Hoffman, R.M., and Nam, J.H. Progression-free survival is accurately predicted in patients treated with chemotherapy for epithelial ovarian cancer by the histoculture drug response assay in a prospective correlative clinical trial at a single institution. Anticancer Res. 33, 1029-1034, 2013.

57.    Mii, S., Duong, J., Tome, Y., Uchugonova, A., Liu, F., Amoh, Y., Saito, N., Katsuoka, K., and Hoffman, R. M. The role of hair follicle nestin-expressing stem cells during whisker sensory-nerve growth in long-term 3D culture. J. Cell. Biochem. 114, 1674-1684, 2013.

58.     Hoffman, R.M. Tissue Culture. In: Brenner’s Encyclopedia of Genetics, 2nd Ed., Vol. 7, pp. 73-76. Elsevier, 2013

59.     Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Uehara, F., Yamamoto, M., Kishimoto, H., Tazawa, H., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging. Cell Cycle 13, 953-960, 2014.

60.     Mii, S., Amoh, Y., Katsuoka, K., and Hoffman, R. M. Comparison of nestin-expressing multipotent stem cells in the tongue fungiform papilla and vibrissa hair follicle. J. Cell. Biochem. 115, 1070-1076, 2014.

61.     Tome, Y., Uehara, F., Mii, S., Yano, S., Zhang, L., Sugimoto, N., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. 3-dimensional tissue is formed from cancer cells in vitro on Gelfoam, but not on MatrigelTM. J. Cell. Biochem., J. Cell. Biochem. 115, 1362-1367, 2014.

62.     Uehara, F., Miwa, S., Tome, Y., Hiroshima, Y., Yano, S., Yamamoto, M., Efimova, E., Matsumoto, Y., Maehara, H., Bouvet, M., Kanaya, F., and Hoffman, R.M. Comparison of UVB and UVC effects on the DNA damage-response protein 53BP1 in human pancreatic cancer. J. Cell. Biochem. 115, 1724-1728, 2014.